Oh, Hyun Ju Gong, Gyeong taek Ahn, Jung Ho Ko, Ja Kyong Lee, Sun-Mi Um, Youngsoon 2024-01-12T02:33:45Z 2024-01-12T02:33:45Z 2022-11-21 2023-01 0960-8524 https://pubs.kist.re.kr/handle/201004/75867 This study achieved high production of hexanol via gas fermentation using Clostridium carboxidivorans P7 by extracting hexanol from the fermentation broth. The hexanol extraction efficiency and inhibitory effects on C. carboxidivorans P7 of 2-butyl-1-octanol, hexyl hexanoate and oleyl alcohol were examined, and oleyl alcohol was selected as the extraction solvent. Oleyl alcohol was added at the beginning of fermentation and during fermentation or a small volume of oleyl alcohol was repeatedly added during fermentation. The addition of a small volume of oleyl alcohol during fermentation was the most effective for CO consumption and hexanol production (5.06 g/L), yielding the highest known hexanol titer through any type of fermentation including gas fermentation. Hexanol production was further enhanced to 8.45 g/L with the repeated addition of oleyl alcohol and ethanol during gas fermentation. The results of this study will enable sustainable and carbon-neutral hexanol production via gas fermentation. English Elsevier BV Effective hexanol production from carbon monoxide using extractive fermentation with Clostridium carboxidivorans P7 Article 10.1016/j.biortech.2022.128201 1 Bioresource Technology, v.367 Bioresource Technology 367 Y scie scopus 000907848800006 Agricultural Engineering Biotechnology & Applied Microbiology Energy & Fuels Agriculture Biotechnology & Applied Microbiology Energy & Fuels Article ETHANOL ALCOHOLS C-2-C-6 Clostridium carboxidivorans P7 Hexanol Syngas Carbon monoxide Acetogen Extractive fermentation