Kim, Seongchan Lee, Ji-Seon 이효진 2024-01-12T02:33:47Z 2024-01-12T02:33:47Z 2022-09-23 2023-01 1385-8947 https://pubs.kist.re.kr/handle/201004/75869 Chemical reagents are widely used to block disease development, including tumor progression and increase in abnormal cell populations; however, their consistent usage is limited owing to drug-related side effects and resistance. Although strategies to enhance the intracellular delivery of anticancer drugs, even at low concen-trations, and decrease their undesired side effects have been developed, practical approaches for liver cancer are still limited. Combination therapy has become a solution for improving chemotherapeutic efficacy. Here, we designed a dual-therapeutic platform by engineering graphene oxide (GO) as a multi-functional co-delivery carrier that targets multiple organelles in cells. It delivers two therapeutic reagents as a single vehicle while simultaneously blocking genes in the nucleus and cytoplasm. We adopted a xenograft mouse model of hepatitis C virus infection to verify the therapeutic efficacy of this combinatorial approach. Our strategy successfully sup-pressed tumor growth by enhancing the intracellular accumulation of anticancer drugs even at one-tenth of the conventional dosage of drugs. Simultaneously, the dual-treat method showed a twofold therapeutic efficiency compared with single-drug treatment. By facilitating a combination of individual pharmacological effects, the present functional GO-based delivery system could be a promising multimodal therapeutic platform owing to its high stability, biocompatibility, and multifunctionality. English Elsevier BV Combination treatment of hepatitis C virus-associated hepatocellular carcinoma by simultaneously blocking genes in multiple organelles via functionally engineered graphene oxide Article 10.1016/j.cej.2022.139279 1 Chemical Engineering Journal, v.452 Chemical Engineering Journal 452 Y scie scopus 000863344600003 Engineering, Environmental Engineering, Chemical Engineering Article IN-VITRO NANOGRAPHENE OXIDE INTRATUMORAL INJECTION ANTICANCER DRUG QUANTUM DOTS CANCER-CELLS DOXORUBICIN NANOPARTICLES DELIVERY THERAPY Graphene oxide Hepatitis C virus Hepatocellular carcinoma Combinatorial drug therapy RNA therapeutics