Baek, Ji Min Jung, Woo Hyuk Eui-Sang Yu Ahn, Dong June Ryu, Yong-Sang 2024-01-12T02:34:34Z 2024-01-12T02:34:34Z 2022-11-16 2022-12 0002-7863 https://pubs.kist.re.kr/handle/201004/75902 Passive water penetration across the cell membrane by osmotic diffusion is essential for the homeostasis of cell volume, in addition to the protein-assisted active transportation of water. Since membrane components can regulate water permeability, controlling compositional variation during the volume regulatory process is a prerequisite for investigating the underlying mechanisms of water permeation and related membrane dynamics. However, the lack of a viable in vitro membrane platform in hypertonic solutions impedes advanced knowledge of cell volume regulation processes, especially cholesterolenriched lipid domains called lipid rafts. By reconstituting the liquid-ordered (Lo) domain as a likeness of lipid rafts, we verified suppressed water permeation across the Lo domains, which had yet to be confirmed with experimental demonstrations despite a simulation approach. With the help of direct transfer of the Lo domains from vesicles to supported lipid membranes, the biological roles of lipid composition in suppressed water translocation were experimentally confirmed. Additionally, the improvement in membrane stability under hypertonic conditions was demonstrated based on molecular dynamics simulations. English American Chemical Society In Vitro Membrane Platform for the Visualization of Water Impermeability across the Liquid-Ordered Phase under Hypertonic Conditions Article 10.1021/jacs.2c06626 1 Journal of the American Chemical Society, v.144, no.48, pp.21887 - 21896 Journal of the American Chemical Society 144 48 21887 21896 N scie scopus 000894220600001 Chemistry, Multidisciplinary Chemistry Article SPANNING LIPID-BILAYERS MOLECULAR-DYNAMICS CHOLESTEROL PERMEABILITY SIMULATIONS SEPARATION RAFTS