Kwon, Haeun Nguyen, Quynh Nhu Ryu, Seung Mok Kwon, Jaeyoung Park, Sojung Guo, Yuanqiang Hwang, Bang Yeon Yim, Joung Han Kim, Jae-Jin Kang, Ki Sung Lee, Dongho 2024-01-12T02:34:37Z 2024-01-12T02:34:37Z 2022-11-15 2022-12 1874-3900 https://pubs.kist.re.kr/handle/201004/75904 Estrogen is a vital hormone responsible for the development of the female reproductive system. Hence, estrogen deficiency in menopause increases various symptoms. This study aimed to discover biologically active com-pounds from an EtOAc-extract of Neopestalotiopsis clavispora culture medium which has previously shown es-trogenic activity. A new alpha-pyrone (1) and a new tetramic acid derivative (2), together with hymenosetin (3) and pestalotiollide B (4), were isolated from the extract. The chemical structures of the new compounds were elucidated using MS and NMR spectroscopy, and the absolute configurations were established by quantum mechanical calculations of electronic circular dichroism and gauge-including atomic orbital NMR chemical shift, followed by DP4 + analysis. The isolated compounds were initially tested for their estrogenic activities using MCF-7 estrogen responsive human breast cancer cells. Hymenosetin (3) showed estrogenic activity by increasing the proliferation of MCF-7 cells at the concentration of 2.5 mu M via the phosphorylation of estrogen receptor-alpha. English Elsevier BV Chemical constituents from Neopestalotiopsis clavispora culture medium with estrogenic effects in MCF-7 cells Article 10.1016/j.phytol.2022.10.008 1 Phytochemistry Letters, v.52, pp.134 - 137 Phytochemistry Letters 52 134 137 N scie scopus 000891910200001 Plant Sciences Chemistry, Medicinal Plant Sciences Pharmacology & Pharmacy Article Neopestalotiopsis clavispora A new ?-pyrone A new tetramic acid derivative Estrogenic activity