Choi, Won Seok Key, Jaehong Youn, In chan LEE, HYO JIN Han, Sungmin 2024-01-12T02:36:50Z 2024-01-12T02:36:50Z 2022-10-12 2022-10 0168-3659 https://pubs.kist.re.kr/handle/201004/75999 Sonothrombolysis with recombinant tissue plasminogen activator (rtPA) and microbubbles has been widely studied to enhance thrombolytic potential. Here, we report different sonothrombolysis strategy in nanoparticles using microbubbles cavitation. We found that different particles in shape exhibited different reactivity toward the cavitation, leading to a distinct sonothrombolytic potential. Two different gold nanoparticles in shape were functionalized with the rtPA: rtPA-functionalized gold nanospheres (NPt) and gold nanostars (NSt). NPt could not accelerate the thrombolytic potential with a sole acoustic stimulus. Importantly, NSt enhanced the potential with acoustic stimulus and microbubble-mediated cavitation, while NPt were not reactive to cavitation. Coad-ministration of NSt and microbubbles resulted in a dramatic reduction of the infarcts in a photothrombotic model and recovery in the cerebral blood flow. Given the synergistic effect and in vivo feasibility of this strategy, cavitation-assisted sonothrombolysis by asymmetrical NSt might be useful for treating acute ischemic stroke. English Elsevier BV Cavitation-assisted sonothrombolysis by asymmetrical nanostars for accelerated thrombolysis Article 10.1016/j.jconrel.2022.09.008 1 Journal of Controlled Release, v.350, pp.870 - 885 Journal of Controlled Release 350 870 885 N scie scopus 000874900400002 Chemistry, Multidisciplinary Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article ACUTE ISCHEMIC-STROKE ULTRASOUND-ENHANCED THROMBOLYSIS GOLD NANOPARTICLES PLASMINOGEN-ACTIVATOR RT-PA T-PA MICROBUBBLES THROMBECTOMY MANAGEMENT FREQUENCY Sonothrombolysis Asymmetrical gold Nanostars Cavitation Synergistic therapeutic effect