Kim, Min Jeong Nho, Chu Won 2024-01-12T02:44:36Z 2024-01-12T02:44:36Z 2023-11-07 2023-10-20 https://pubs.kist.re.kr/handle/201004/76357 Physalin A (PA), the compound derived from Physalis alkekengi has been reported for various pharmacological properties including antioxidant, anti-inflammation activities. However, anti-obesity effect of Physalis alkekengi water extract (PAE) and PA has not been reported yet. In this study, we aimed to examine the anti-obesity effect of PAE and PA in 3T3-L1 cells. Cell viability assay was conducted using PAE and PA in 3T3-L1 cells and no significant cytotoxicity was observed below 160 μg/ml of PAE and 5 μM of PA. By treating PAE and PA, we measured the TG contents from cell supernatant media and observed a dose-dependent inhibition for lipid accumulation using TG assay and Oil red O staining. In addition, protein and mRNA expression of related key factors for adipogenesis and lipolysis were measured by western blotting and qRT-PCR in PAE and PA treated 3T3-L1 cells. Especially, PA decreased mRNA expression level of adipogenesis-related genes and regulated lipolysis-related genes in 3T3-L1 cells. These results suggest that PAE and its active compound, PA could be considered as potential candidates of nutraceuticals and natural medicine for prevention of obesity. English The Korean Society of Food Science and Nutrition Anti-obesity Effect of Physalis Alkekengi in 3T3-L1 Cells through Inhibition of Adipogenesis and Activation of Lipolysis Conference 1 2023 KFN International Symposium and Annual Meeting 2023 KFN International Symposium and Annual Meeting KO BEXCO, Busan, Korea 2023-10-18 Sustainable Food System for a Healthy & Value-Oriented Life