Lee, Wonjun Park, Jiin Kang, Dongil Suh, Seungbeum 2024-01-12T02:47:30Z 2024-01-12T02:47:30Z 2023-06-08 2023-01-17 1084-6999 https://pubs.kist.re.kr/handle/201004/76501 This paper reports a microfluidic platform tailored for emulating fundamentals of bacteria-mediated cancer therapy (BMCT). Specifically, we attempted to replicate the basic features of the bacteria-colonized tumor microenvironment and the accompanying immune response in a single well of the device. The effects of bacterial infection or stimulation on tumor spheroids were observed to experimentally examine bacteria's innate antitumor cytotoxicity and potential as a therapeutic agent carrier in vitro. Consequently, we observed enhanced dead signals as well as decreased stemness of tumor spheroids as a result of bacterial cytotoxicity and IFN-beta secretion, respectively. Furthermore, enzyme-linked immunosorbent assay results from cell culture media indicative of co-cultured macrophage's M1 polarization demonstrated bacteria's potential for immune-activating adjuvant. Our device, with a user-friendly platform leveraging spontaneous capillary, will offer a chance to scrutinize BMCT at cellular and tissue levels. English IEEE Reconstituting Fundamentals of Bacteria Mediated Cancer Therapy On A Chip Conference 10.1109/MEMS49605.2023.10052432 1 36th IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp.91 - 94 36th IEEE International Conference on Micro Electro Mechanical Systems (MEMS) 91 94 US Munich, GERMANY 2023-01-15 2023 IEEE 36TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, MEMS 000975359200026 2-s2.0-85149866601