Lee, Seung Mi Park, Kyo Hoon Joo, Eunwook Jeong, Da Eun Lee, Ji Eun Lee, Kyong-No Shin, Sue 2024-01-12T03:00:11Z 2024-01-12T03:00:11Z 2022-07-20 2022-09 1046-7408 https://pubs.kist.re.kr/handle/201004/76620 Problem To identify potential proteins in the amniotic fluid (AF) that may be associated with histologic chorioamnionitis (HCA) in patients with preterm premature rupture of membranes (PPROM) using antibody-based microarray analysis. Method of study This was a retrospective cohort study involving 100 singleton pregnant women with PPROM at 24-34 weeks who underwent amniocentesis and delivered within 120 h of amniocentesis. First, the AF proteomes of 15 patients with PPROM and HCA were compared with those of 15 gestational age-matched patients without HCA using a protein microarray. Next, 12 candidate proteins associated with HCA were further validated in 100 consecutive patients with PPROM by ELISA. Results Of 507 proteins assessed in the microarray analysis, 46 showed significant intergroup differences. Further quantification confirmed that the levels of EN-RAGE, IL-6, MMP-9, TNFR2, SPARC, TSP2, and uPA were higher in the AF of PPROM patients with HCA than in those without. Multivariate analyses also showed that elevated AF EN-RAGE, IL-6, MMP-9, and TNFR2 levels were independently associated with HCA when adjusted for baseline variables. The frequency of the highest quartile of the aforementioned proteins significantly increased as the total grade of HCA increased; the risk of HCA significantly increased with increasing AF levels of each protein (P for trend < .001). Conclusions Using protein-antibody microarray technology, we discovered several potential AF proteins (EN-RAGE, IL-6, MMP-9, and TNFR2) independently associated with HCA in patients with PPROM. Furthermore, we demonstrated a direct correlation between the gradation of the intra-amniotic inflammatory response and HCA severity. English Blackwell Publishing Inc. High-throughput analysis of amniotic fluid proteins associated with histological chorioamnionitis in preterm premature rupture of membranes using an antibody-based microarray Article 10.1111/aji.13595 1 American Journal of Reproductive Immunology, v.88, no.3 American Journal of Reproductive Immunology 88 3 N scie scopus 000822582700001 Immunology Reproductive Biology Immunology Reproductive Biology Article TUMOR-NECROSIS-FACTOR PRELABOR RUPTURE INTRAAMNIOTIC INFLAMMATION PLASMINOGEN-ACTIVATOR CYTOKINE LEVELS FACTOR-ALPHA RISK-FACTOR RECEPTOR FETAL INTERLEUKIN-6 amniotic fluid antibody microarray histologic chorioamnionitis proteins preterm premature rupture of membranes