Byun Gyuwon Kim Soh-Youn Choi Min-Woo Yang, Jin Kyoung Kwon, Ji Eon Kim Sehoon Park Soo Young 2024-01-12T03:00:33Z 2024-01-12T03:00:33Z 2022-10-28 2022-08 0143-7208 https://pubs.kist.re.kr/handle/201004/76632 High-performance microscopy with organelle-targeting fluorescent probes has been an indispensable bioimaging tool for cell biology. Confocal or super-resolution imaging often requires highly magnified and/or longitudinal scanning conditions, necessitating the advent of photostable fluorophores. Herein, we report novel organelle targeting fluorescent probes derived from a highly photostable fluorophoric skeleton, i.e., 1,5-naphthyridine2,6-dione (NTD). With organelle-specific derivatization and pi-conjugation extention, we developed NTD-based molecular probes with blue-to-red fluorescence tunability and intracellular organelle selectivity for staining, as well as minimal cytotoxicity, which allowed us to demonstrate intracellular differentiation of lysosomes and mitochondria by multi-color confocal imaging. Under the condition of longitudinal microscopic examination, these new probes showed excellent photostability of fluorescent signals as opposed to the rapid photobleaching of commercial trackers. By virtue of these beneficial properties, the NTD-based probes were successfully applied to super-resolution cell imaging by enduring the high-power illumination conditions of sub-diffraction microscopic techniques including stimulated emission depletion (STED) and structured illumination microscopy (SIM). English Elsevier BV Highly photostable fluorescent probes for multi-color and super-resolution imaging of cell organelles Article 10.1016/j.dyepig.2022.110427 1 Dyes and Pigments, v.204 Dyes and Pigments 204 Y scie scopus 000806792100003