Jeong, Se Yun Na, Myung woo Park, Eon Chung Kim, Jin-Chul Kang, Dong-Min Hamishehkar, Hamed Ahn, Mi-Jeong Kim, Jung Kyu Kim, ki-Hyun 2024-01-12T03:00:38Z 2024-01-12T03:00:38Z 2022-09-02 2022-08 2470-1343 https://pubs.kist.re.kr/handle/201004/76635 Pinus eldarica is a medicinal tree used in traditional herbal medicine for the treatment of bronchial asthma and various skin diseases. As part of our ongoing search for bioactive phytochemicals with novel structures in natural products, we performed a phytochemical analysis of the methanol (MeOH) extract from P. eldarica needles collected in Iran. Phytochemical investigation of the MeOH extract, aided by liquid chromatography-mass spectrometry-based analysis, resulted in the isolation and identification of three labdane-type diterpenes (1-3), including a new and relatively unique norlabdane-type diterpene with a peroxide moiety, eldaricoxide A (1). The chemical structures of the isolated labdane-type diterpenes were elucidated by analyzing the spectroscopic data from 1D and 2D NMR and high-resolution electrospray ionization-mass spectrometry. The absolute configuration of eldaricoxide A (1) was established by employing a computational method, including electronic circular dichroism calculation and specific optical rotation. An anti-Helicobacter pylori test was conducted, where compound 3 exhibited the most potent antibacterial activity against H. pylori strain 51, inducing 72.7% inhibition (MIC50 value of 92 mu M), whereas eldaricoxide A (1) exhibited moderate antibacterial activity against H. pylori strain 51, inducing 54.5% inhibition (MIC50 value of 95 mu M). These findings demonstrated that the identified bioactive labdane-type diterpenes 1 and 3 can be applied in the development of novel antibiotics against H. pylori for the treatment of gastric and duodenal ulcers. English ACS Publications Labdane-type Diterpenes from Pinus eldarica Needles and Their Anti-Helicobacter pylori Activity Article 10.1021/acsomega.2c04147 1 ACS OMEGA, v.7, no.33, pp.29502 - 29507 ACS OMEGA 7 33 29502 29507 Y scie scopus 000849862100001 Chemistry, Multidisciplinary Chemistry Article ESSENTIAL OIL EXTRACT MEDW. INHIBITION UREASE