HEO, JEONG MIN Joung, Chanmin Pahk, Kisoo Pahk, Ki Joo 2024-01-12T03:00:40Z 2024-01-12T03:00:40Z 2022-08-31 2022-08 2045-2322 https://pubs.kist.re.kr/handle/201004/76636 Boiling histotripsy (BH) is a promising High-Intensity Focused Ultrasound technique that can be employed to mechanically fractionate solid tumours. Whilst studies have shown the feasibility of BH to destroy liver cancer, no study has reported on the healing process of BH-treated liver tissue. We therefore extensively investigated the evolution of the healing response of liver to BH in order to provide an insight into the healing mechanisms. In the present study, 14 Sprague Dawley rats underwent the BH treatment and were sacrificed on days 0, 3, 7, 14, and 28 for morphological, histological, serological and qPCR analyses. The area of the treated region was 1.44 cm(2) (1.2 cm x 1.2 cm). A well-defined BH lesion filled with coagulated blood formed on day 0. A week after the treatment, fibroblast activation was induced at the treatment site, leading to the formation of extracellular matrix structure (ECM). The ECM was then disrupted for 7 to 28 days. Regenerated normal hepatocytes and newly formed blood vessels were found within the BH region with the absence of hepatic fibrosis. No significant morphological, histological and genetic changes around the BH lesion occurred. These results suggest that BH could be a safe and promising therapeutic tool for treating solid tumours without inducing any significant adverse effect such as the formation of liver fibrosis. English Nature Publishing Group Investigation of the long-term healing response of the liver to boiling histotripsy treatment in vivo Article 10.1038/s41598-022-18544-7 1 Scientific Reports, v.12, no.1, pp.14462 Scientific Reports 12 1 14462 Y scie scopus 000845011100051 Multidisciplinary Sciences Science & Technology - Other Topics Article INTENSITY FOCUSED ULTRASOUND TISSUE MYOFIBROBLAST MECHANISMS THERAPY CANCER REPAIR