Ahn, Yong tae Park, Sanghyun Ji, Min-Kyu Ha, Geon-Soo Jeon, Byong-Hun Choi, Jaeyoung 2024-01-12T03:01:54Z 2024-01-12T03:01:54Z 2022-04-25 2022-07 0301-4797 https://pubs.kist.re.kr/handle/201004/76683 The adaptability and biofuel production potential of two strains of microalgae isolated and cultivated in livestock wastewater effluent (LWE) with acid mine drainage (AMD) were investigated. The isolated strains of microalgae from samples obtained from LWE and AMD, two microalgal strains (Nephroselmis sp. KGE2 and Autodesmus obliquus KGE17) were selected based on their growth rate and lipid productivity. The dry cell weight of Nephroselmis sp. KGE2 and Autodesmus obliquus KGE17 after 20 days of cultivation in AMD increased from 0.05 to 0.59 g/L and from 0.05 to 0.55 g/L, respectively. These findings revealed a significant accumulation of fatty acids with increasing AMD content. Nephroselmis sp. KGE2 in LWE with 5% AMD demonstrated a higher growth rate (0.59 +/- 0.03 g/L) and fatty acid production (401.5 +/- 47.3 mg/L) than Autodesmus obliquus KGE17 with 5% AMD. Additionally, Nephroselmis sp. KGE2 had C16-C18 fatty acid content (92.4%) in LWE with AMD. Biodiesel produced from Nephroselmis sp. KGE2 had a higher cetane number (52.31) and iodine value (88.26 g I2/100 g oil). Consequently, Nephroselmis sp. KGE2 can be considered a potential candidate for biodiesel production using AMD as an iron source. English Academic Press Biodiesel production potential of microalgae, cultivated in acid mine drainage and livestock wastewater Article 10.1016/j.jenvman.2022.115031 1 Journal of Environmental Management, v.314, pp.115031 Journal of Environmental Management 314 115031 N scie scopus 000804381000007 Environmental Sciences Environmental Sciences & Ecology Article LIPID-ACCUMULATION IRON BIOMASS GROWTH NUTRIENT REMOVAL OXIDATION METALS Microalgae Biodiesel Acid mine drainage Livestock wastewater effluent