Lee Mijin Youn Esther Kang Kyungsu Shim Yhong-Hee 2024-01-12T03:30:54Z 2024-01-12T03:30:54Z 2022-08-08 2022-05 2076-3921 https://pubs.kist.re.kr/handle/201004/76725 In recent decades, maternal age at first birth has increased, as has the risk of infertility due to rapidly declining oocyte quality with age. Therefore, an understanding of female reproductive aging and the development of potential modulators to control oocyte quality are required. In this study, we investigated the effects of 3,3'-diindolylmethane (DIM), a natural metabolite of indole-3-cabinol found in cruciferous vegetables, on fertility in a Caenorhabditis elegans model. C. elegans fed DIM showed decreased mitochondrial dysfunction, oxidative stress, and chromosomal aberrations in aged oocytes, and thus reduced embryonic lethality, suggesting that DIM, a dietary natural antioxidant, improves oocyte quality. Furthermore, DIM supplementation maintained germ cell apoptosis (GCA) and germ cell proliferation (GCP) in a CEP-1 /p53-dependent manner in a reproductively aged C. elegans germ line. DIM-induced GCA was mediated by the CEP-1-EGL-1 pathway without HUS-1 activation, suggesting that DIM-induced GCA is different from DNA damage-induced GCA in the C. elegans germ line. Taken together, we propose that DIM supplementation delays the onset of reproductive aging by maintaining the levels of GCP and GCA and oocyte quality in a reproductively aged C. elegans. English MDPI AG 3,3 '-Diindolylmethane Supplementation Maintains Oocyte Quality by Reducing Oxidative Stress and CEP-1/p53-Mediated Regulation of Germ Cells in a Reproductively Aged Caenorhabditis elegans Model Article 10.3390/antiox11050950 1 Antioxidants, v.11, no.5, pp.950 Antioxidants 11 5 950 Y scie scopus 000803411500001 Biochemistry & Molecular Biology Chemistry, Medicinal Food Science & Technology Biochemistry & Molecular Biology Pharmacology & Pharmacy Food Science & Technology Article CHROMOSOME SEGREGATION CRUCIFEROUS VEGETABLES MEIOTIC MATURATION LIFE-SPAN MITOCHONDRIAL PROTEIN APOPTOSIS DAMAGE DIM PROLIFERATION Caenorhabditis elegans 3,3 &apos -diindolylmethane antioxidant reproductive aging oocyte quality CEP-1/p53 germ cell apoptosis germ cell proliferation mitochondrial function oxidative stress