하태웅 김미선 Kang, Bok Eum 김경민 Seong Su Hong Kang, Taek JunHyuk Woo Han, Kyung reem Oh, Uh taek Chun Whan Choi Hong, Gyu-Sang 2024-01-12T03:31:48Z 2024-01-12T03:31:48Z 2022-03-29 2022-04 0021-8561 https://pubs.kist.re.kr/handle/201004/76760 Several studies have documented the broad-spectrum bioactivities of a lotus seed (Plumula nelumbinis [PN]) green embryo extract. However, the specific bioactive components and associated molecular mechanisms remain largely unknown. This study aimed to identify the ion channel-activating mechanisms of PN extracts. Using fluorometric imaging and patch-clamp recordings, PN extracts were screened for calcium channel activation in dorsal root ganglion (DRG) neurons. The TRPV1 channels in DRG neurons were strongly activated by the PN extract (mean amplitude of 131 ± 45 pA at 200 μg/mL) and its purified glycosyloxyflavone narcissoside (401 ± 271 pA at 100 μM). Serial treatment with a 200 μg/mL PN extract in TRPV1-overexpressing HEK293T cells induced robust desensitization to 10 ± 10% of the initial current amplitude. Thus, we propose that the PN extract and narcissoside function as TRPV1 agonists. This new finding may advance our knowledge regarding the traditional and scientific functions of PN in human health and disease English American Chemical Society Lotus Seed Green Embryo Extract and a Purified Glycosyloxyflavone Constituent, Narcissoside, Activate TRPV1 Channels in Dorsal Root Ganglion Sensory Neurons Article 10.1021/acs.jafc.1c07724 1 Journal of Agricultural and Food Chemistry, v.70, no.13, pp.3969 - 3978 Journal of Agricultural and Food Chemistry 70 13 3969 3978 N scie scopus 000786591900004 Agriculture, Multidisciplinary Chemistry, Applied Food Science & Technology Agriculture Chemistry Food Science & Technology Article PHENOLIC-COMPOUNDS FORCE-FIELD RECEPTOR OPTIMIZATION ANTIOXIDANT FLAVONOIDS GLYCOSIDES SPEED green embryo of lotus seed narcissoside TRPV1 ion channel sensory neuron