Lee Yeonjung Tran, Nguyen Hoang Phuong 고자경 Gong, Gyeong taek Um, Young soon 한성옥 Lee, Sun Mi 2024-01-12T03:32:42Z 2024-01-12T03:32:42Z 2022-02-25 2022-02 2296-4185 https://pubs.kist.re.kr/handle/201004/76804 Efficient xylose catabolism in engineered Saccharomyces cerevisiae enables more economical lignocellulosic biorefinery with improved production yields per unit of biomass. Yet, the product profile of glucose/xylose co-fermenting S. cerevisiae is mainly limited to bioethanol and a few other chemicals. Here, we introduced an n-butanol-biosynthesis pathway into a glucose/xylose co-fermenting S. cerevisiae strain (XUSEA) to evaluate its potential on the production of acetyl-CoA derived products. Higher n-butanol production of glucose/xylose co-fermenting strain was explained by the transcriptomic landscape, which revealed strongly increased acetyl-CoA and NADPH pools when compared to a glucose fermenting wild-type strain. The acetate supplementation expected to support acetyl-CoA pool further increased n-butanol production, which was also validated during the fermentation of lignocellulosic hydrolysates containing acetate. Our findings imply the feasibility of lignocellulosic biorefinery for producing fuels and chemicals derived from a key intermediate of acetyl-CoA through glucose/xylose co-fermentation. English Frontiers Research Foundation Glucose/Xylose Co-Fermenting Saccharomyces cerevisiae Increases the Production of Acetyl-CoA Derived n-Butanol From Lignocellulosic Biomass Article 10.3389/fbioe.2022.826787 1 Frontiers in Bioengineering and Biotechnology, v.10 Frontiers in Bioengineering and Biotechnology 10 Y scie scopus 000764313400001 2-s2.0-85125661427 Biotechnology & Applied Microbiology Multidisciplinary Sciences Biotechnology & Applied Microbiology Science & Technology - Other Topics Article GENETICALLY-ENGINEERED STRAIN BETA-OXIDATION CYCLE XYLOSE FUELS ETHANOL BIOETHANOL COENZYME REVERSAL ACETATE Saccharomyces cerevisiae glucose xylose co-fermentation n-butanol acetyl-CoA acetate lignocellulosic biomass