Kim, Jung-Hwan Park, Tae-Jin Park, Jin-Soo Kim, MinSeon Chi, Won-Jae Kim, Seung-Young 2024-01-12T03:32:59Z 2024-01-12T03:32:59Z 2022-03-08 2021-12 1420-3049 https://pubs.kist.re.kr/handle/201004/76816 Luteolin (LT), present in most plants, has potent anti-inflammatory properties both in vitro and in vivo. Furthermore, some of its derivatives, such as luteolin-7-O-glucoside, also exhibit anti-inflammatory activity. However, the molecular mechanisms underlying luteolin-3 PRIME;-O-phosphate (LTP)-mediated immune regulation are not fully understood. In this paper, we compared the anti-inflammatory properties of LT and LTP and analyzed their molecular mechanisms of action; we obtained LTP via the biorenovation of LT. We investigated the anti-inflammatory activities of LT and LTP in macrophage RAW 264.7 cells. We confirmed from previously reported literature that LT inhibits the production of nitric oxide and prostaglandin E-2, as well as the expression of inducible NO synthetase and cyclooxygenase-2. In addition, expressions of inflammatory genes and mediators, such as tumor necrosis factor-alpha, interleukin-6, and interleukin-1 beta, were suppressed. LTP showed anti-inflammatory activity similar to LT, but better anti-inflammatory activity in all the experiments, while also inhibiting mitogen-activated protein kinase and nuclear factor-kappa B more effectively than LT. At a concentration of 10 mu M, LTP showed differences of 2.1 to 44.5% in the activity compared to LT; it also showed higher anti-inflammatory activity. Our findings suggest that LTP has stronger anti-inflammatory activity than LT. English Multidisciplinary Digital Publishing Institute (MDPI) Luteolin-3'-O-Phosphate Inhibits Lipopolysaccharide-Induced Inflammatory Responses by Regulating NF-kappa B/MAPK Cascade Signaling in RAW 264.7 Cells Article 10.3390/molecules26237393 1 Molecules, v.26, no.23 Molecules 26 23 Y scie scopus 000734579300001 2-s2.0-85121119438 Biochemistry & Molecular Biology Chemistry, Multidisciplinary Biochemistry & Molecular Biology Chemistry Article NITRIC-OXIDE SYNTHASE COX-2 EXPRESSION GENE-EXPRESSION TNF-ALPHA LUTEOLIN PHOSPHORYLATION MACROPHAGES PATHWAY TRANSLOCATION ACTIVATION luteolin-3 &apos -O-phosphate anti-inflammatory MAPK NF-kappa B biorenovation