Park, Yejin Kim, Hyejin Song, Jaeyoon Kim, Sehyeon Lee, Byung Chul Kim, Jinsik 2024-01-12T06:30:17Z 2024-01-12T06:30:17Z 2023-12-12 2024-02 0956-5663 https://pubs.kist.re.kr/handle/201004/79662 Graphene oxide (GO) has many advantages, making it suitable for various applications. However, it has low electrical conductivity, restricting its applicability to electrochemical biosensors. This study used dielectrophoretic (DEP) force to control the movement and deformation of GO nanosheets to achieve high electrical conductivity without the chemical reduction of oxygen functional groups. Subjecting the DEP force to GO nanosheets induced physical deformation leading to the formation of wrinkled structures. A computational simulation was performed to set an appropriate electrical condition for operating a positive DEP force effect of at least 1019 v2/m3, and the interdigitated microelectrode structure was selected. The resulting wrinkled GO exhibited significantly improved electrical conductivity, reaching 21.721 μS while preserving the essential oxygen functional groups. Furthermore, a biosensor was fabricated using wrinkled GO deposited via DEP force. The biosensor demonstrated superior sensitivity, exhibiting a 9.6-fold enhancement compared with reduced GO (rGO) biosensors, as demonstrated through biological experiments targeting inducible nitric oxide synthase. This study highlights the potential of using DEP force to enhance electrical conductivity in GO-based biosensing applications, opening new avenues for high-performance diagnostics. English Pergamon Press Ltd. Dielectrophoretic force-induced wrinkling of graphene oxide: Enhancing electrical conductivity and expanding biosensing applications Article 10.1016/j.bios.2023.115867 1 Biosensors and Bioelectronics, v.246 Biosensors and Bioelectronics 246 N scie scopus 001140089400001 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article NANOSTRUCTURES REDUCTION BIOMARKER SYNTHASE Dielectrophoretic force Wrinkles Graphene oxide Chemical reduction Electrical conductivity Biosensor