Lim, Songhyun JUNG HEERA Lee, Hyelim Chu, Yeonjeong Kim, Hyejin Kim, Eunha Lee, Sanghee 2024-01-12T06:31:08Z 2024-01-12T06:31:08Z 2023-12-14 2023-12 0753-3322 https://pubs.kist.re.kr/handle/201004/79688 The stimulator of the interferon gene (STING) signaling pathway acts as a primary defense system against DNA pathogens. Because of the crucial role of STING in type I interferon (IFN) response and innate immunity, extensive research has been conducted to elucidate the roles of various effector molecules involved in STING-mediated signal transduction. However, despite the substantial contribution of microtubules to the immune system, the association between the STING signaling pathway and microtubules remains unclear. In this study, we revealed that the modulation of STING via microtubule-destabilizing agents (MDAs) specifically induced type I IFN responses rather than inflammatory responses in human monocytes. Co-treatment of MDAs with STING agonists induced the elevation of phospho-TANK-binding kinase 1 (TBK1), amplifying the innate immune response. However, during the deficiency of TBK1, the non-canonical signaling pathway through nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) contributed to MDA-induced STING activation in type I IFN response which suggested the versatile regulation of MDA in STING-mediated immunity. English Elsevier Masson Microtubule-destabilizing agents enhance STING-mediated innate immune response via biased mechanism in human monocyte cells Article 10.1016/j.biopha.2023.115883 1 Biomedicine & Pharmacotherapy, v.169 Biomedicine & Pharmacotherapy 169 Y scie scopus 001115921000001 Medicine, Research & Experimental Pharmacology & Pharmacy Research & Experimental Medicine Pharmacology & Pharmacy Article CYCLIC GMP-AMP DNA SENSOR IN-VIVO PATHWAY LAULIMALIDE PACLITAXEL POTENT STABILIZATION NOCODAZOLE ACTIVATION Stimulator of interferon genes (STING) Microtubule Type I interferon response Pro-inflammatory response