Yecheol Bak TIKUM FLORENCE ANJONG Park, Jihwan Jeon, Sang Hyeon Kim, ji hwan Lee, Kangwon Shin, Iksoo Kim, Sehoon 2024-01-12T06:32:45Z 2024-01-12T06:32:45Z 2023-11-17 2023-11 0925-4005 https://pubs.kist.re.kr/handle/201004/79764 Hydrogen sulfide (H2S), an antioxidant and cell signaling molecule, plays a critical role in redox reactions in the biological system, and its imbalances in blood levels have been implicated in various diseases. Here, we demonstrate a novel chemosensor-based “turn-on” electrochemiluminescence (ECL) system for the selective and sensitive detection of H2S in a type 1 diabetes rat model. Our luminogenic chemosensor (Probe) is a redox-stable cyclometalating iridium (III) complex with emission-quenching 2,4-dinitrophenyl ether (DNP) that shows a significant enhancement in emission intensity upon cleavage of DNP by selective reaction towards H2S. To achieve robust and reliable ECL emission of Probe for biological analysis, we developed a facile deproteinization protocol based on organic solvent-induced protein precipitation/syringe filtration that eliminates uncontrolled protein adsorption on the electrode surface from protein-rich biological samples. Our deproteinization-assisted ECL chemosensor system showed effectiveness in detecting disease-associated H2S levels in biological media with a linear response in the range of 0？250 ？M (R2: 0.992). It enables reliable in vitro monitoring of H2S level changes in blood samples of a type 1 diabetes rat model (P = 0.0296). This strategy to integrate chemosensor-based ECL and facile deproteinization protocol provides a sensitive and rapid disease-monitoring platform for detecting small-molecule biomarkers. English Elsevier BV Monitoring disease-implicated hydrogen sulfide in blood using a facile deproteinization-based electrochemiluminescence chemosensor system Article 10.1016/j.snb.2023.134364 1 Sensors and Actuators, B: Chemical, v.394 Sensors and Actuators, B: Chemical 394 N scie scopus 001144437900001 Chemistry, Analytical Electrochemistry Instruments & Instrumentation Chemistry Electrochemistry Instruments & Instrumentation Article FLUORESCENT-PROBE QUANTITATIVE DETECTION PROTEIN ADSORPTION SIGNALING MOLECULE RAPID DETECTION QUANTUM DOTS COMPLEXES SENSOR GASOTRANSMITTER STREPTOZOTOCIN Electrochmiluminescence Chemosensor Hydrogen sulfide Small molecule biomarker Type 1 diabetes