Jeon, Byeong Jun Yoo, Nayeon Kim, Jeong Do Choi, Jaeyoung 2024-01-12T06:33:29Z 2024-01-12T06:33:29Z 2023-10-26 2023-10 1664-462X https://pubs.kist.re.kr/handle/201004/79800 The genus Streptomyces has been unceasingly highlighted for the versatility and diversity of the antimicrobial agents they produce. Moreover, it is a heavily sequenced taxon in the phylum Actinobacteria. In this study, 47 sequence profiles were identified as proteins highly conserved within the genus Streptomyces. Significant hits to the 38 profiles were found in more than 2000 Streptomyces genomes, 11 of which were further conserved in more than 90% of Actinobacterial genomes analyzed. Only a few genes corresponding to these sequence profiles were functionally characterized, which play regulatory roles in the morphology and biosynthesis of antibiotics. Here a highly conserved sequence, namely, SHC-AMP (Streptomyces highly conserved antimicrobial peptide), which exhibited antimicrobial activity against bacterial and fungal plant pathogens, was reported. In particular, Arabidopsis thaliana was effectively protected against infection with Pseudomonas syringae pv. tomato DC3000 by treatment with this peptide. Results indicated the potential application of this peptide as an antimicrobial agent for control of plant diseases. Our results suggest putative target genes for controlling Streptomyces spp., including the one exhibiting antimicrobial activity against a wide range of phytopathogens. English Frontiers Media S.A. A peptide encoded by a highly conserved gene belonging to the genus Streptomyces shows antimicrobial activity against plant pathogens Article 10.3389/fpls.2023.1250906 1 Frontiers in Plant Science, v.14 Frontiers in Plant Science 14 Y scie scopus 001089203500001 Plant Sciences Plant Sciences Article NATURAL-PRODUCTS ANTIBACTERIAL METABOLITES SEQUENCES DATABASE THIONIN antimicrobial peptide Streptomyces plant pathogen highly conserved sequence antimicrobial activity