Lee, Hyeon-Jeong Jin, Jonghwa Seo, Yoondam Kang, Inseon SON, Jung hyun Yi, Eugene C Min, Ho phil 2024-01-12T06:34:21Z 2024-01-12T06:34:21Z 2023-09-22 2023-09 2305-6304 https://pubs.kist.re.kr/handle/201004/79840 Di-(2-Ethylhexyl) phthalate (DEHP) is a prevalent environmental endocrine disruptor that affects homeostasis, reproduction, and developmental processes. The effects of DEHP have been shown to differ based on sex and sexual maturity. This study examines the metabolic profiles of mature adult rats from both sexes, aged 10 weeks, and adolescent female rats, aged 6 weeks, following a single 5 mg/kg of body weight DEHP oral administration. An untargeted metabolomic analysis was conducted on urine samples collected at multiple times to discern potential sex- and maturity-specific DEHP toxicities. Various multivariate statistical analyses were employed to identify the relevant metabolites. The findings revealed disruptions to the steroid hormone and primary bile acid biosynthesis. Notably, DEHP exposure increased hyocholic, muricholic, and ketodeoxycholic acids in male rats. Moreover, DEHP exposure was linked to heart, liver, and kidney damage, as indicated by increased plasma GOT1 levels when compared to the levels before DEHP exposure. This study provides detailed insights into the unique mechanisms triggered by DEHP exposure concerning sex and sexual maturity, emphasizing significant distinctions in lipid metabolic profiles across the different groups. This study results deepens our understanding of the health risks linked to DEHP, informing future risk assessments and policy decisions. English MDPI AG Untargeted Metabolomics Analysis Reveals Toxicity Based on the Sex and Sexual Maturity of Single Low-Dose DEHP Exposure Article 10.3390/toxics11090794 1 Toxics, v.11, no.9 Toxics 11 9 Y scie scopus 001097109400001 Environmental Sciences Toxicology Environmental Sciences & Ecology Toxicology Article DI(2-ETHYLHEXYL)PHTHALATE PUBERTY AGE DEHP metabolomics multivariate statistical analysis LC-MS/MS low dose