Nam, Yunseong Kim, Myung Suk Erdenebileg, Saruul Cha, Kwang Hyun Ryu, Da Hye Kim, Ho Youn Lee, Suhyun JUNG, JE HYEONG Nho, Chu Won 2024-01-12T06:34:22Z 2024-01-12T06:34:22Z 2023-09-11 2023-09 2072-6643 https://pubs.kist.re.kr/handle/201004/79841 Non-alcoholic fatty liver disease (NAFLD) is a leading cause of chronic liver diseases and encompasses non-alcoholic steatosis, steatohepatitis, and fibrosis. Sanguisorba officinalis L. (SO) roots have traditionally been used for their antioxidant properties and have beneficial effects on metabolic disorders, including diabetes and obesity. However, its effects on hepatic steatosis and fibrosis remain unclear. In this study, we explored the effects of a 95% ethanolic SO extract (SOEE) on NAFLD and fibrosis in vivo and in vitro. The SOEE was orally administered to C57BL/6J mice fed a choline-deficient, L-amino-acid-defined, high-fat diet for 10 weeks. The SOEE inhibited hepatic steatosis by modulating hepatic malondialdehyde levels and the expression of oxidative stress-associated genes, regulating fatty-acid-oxidation-related genes, and inhibiting the expression of genes that are responsible for fibrosis. The SOEE suppressed the deposition of extracellular matrix hydroxyproline and mRNA expression of fibrosis-associated genes. The SOEE decreased the expression of fibrosis-related genes in vitro by inhibiting SMAD2/3 phosphorylation. Furthermore, the SOEE restored the gut microbial diversity and modulated specific bacterial genera associated with NAFLD and fibrosis. This study suggests that SOEE might be the potential candidate for inhibiting hepatic steatosis and fibrosis by modulating oxidative stress, fatty acid oxidation, and gut microbiota composition. English Multidisciplinary Digital Publishing Institute (MDPI) Sanguisorba officinalis L. Ameliorates Hepatic Steatosis and Fibrosis by Modulating Oxidative Stress, Fatty Acid Oxidation, and Gut Microbiota in CDAHFD-Induced Mice Article 10.3390/nu15173779 1 Nutrients, v.15, no.17 Nutrients 15 17 Y scie scopus 001064162100001 Nutrition & Dietetics Nutrition & Dietetics Article PEROXISOMAL BETA-OXIDATION ACTIVATED RECEPTOR-ALPHA STELLATE CELLS NONALCOHOLIC STEATOHEPATITIS LIVER-DISEASE EXTRACELLULAR-MATRIX TGF-BETA IN-VITRO GROWTH INFLAMMATION Sanguisorba officinalis L. steatosis fibrosis gut microbiota choline-deficient L-amino acid-defined high-fat diet (CDAHFD)