Heo, Cheol Ho Bak, Seon Young 김용한 옥명렬 Kim, So Yeon 2024-01-12T06:34:51Z 2024-01-12T06:34:51Z 2023-06-11 2023-08 1742-7061 https://pubs.kist.re.kr/handle/201004/79865 Integrin-mediated focal adhesion (FA) and subsequent cytoskeletal reorganization influence cell morphology, migration, and ultimately cell fate. Previous studies have used various patterned surfaces with defined macroscopic cell shapes or nanoscopic FA distributions to explore how different substrates affect the fate of human bone marrow mesenchymal stem cells (BMSCs). However, there is currently no straightforward relationship between BMSC cell fates induced by patterned surfaces and FA distribution substrates. In this study, we conducted single-cell image analysis of integrin αv-mediated FA and cell morphological features of BMSCs during biochemically induced differentiation. This enabled the identification of distinct FA features that can discriminate between osteogenic and adipogenic differentiation, demonstrating that integrin αv-mediated focal adhesion (FA) can be used as a non-invasive biomarker for real time observation. Based on these results, we developed an organized microscale fibronectin (FN) patterned surface where the fate of BMSC could be precisely manipulated by these FA features. Notably, even in the absence of any biochemical inducers, such as those contained in the differentiation medium, BMSCs cultured on these FN patterned surfaces exhibited upregulation of differentiation markers comparable to BMSCs cultured using conventional differentiation methods. Therefore, the present study reveals the application of these FA features as universal markers not only for predicting differentiation status, but also for regulating cell fate by precisely controlling the FA features with a new cell culture platform. English Elsevier BV Development of an integrin αv-based universal marker, capable of both prediction and direction of stem cell fate Article 10.1016/j.actbio.2023.04.044 1 Acta Biomaterialia, v.166, pp.291 - 300 Acta Biomaterialia 166 291 300 N scie scopus 001033901300001 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article MATRIX INTERACTIONS DIFFERENTIATION SHAPE Integrin-mediated focal adhesion Integrin a(v) Image analysis Patterned microwell array Cell fate