Mahmud, Joyeta HIEN, THI MY ONG 에다 아테스 Seo, Hong Seog Kang, Min-Jung 2024-01-12T06:35:37Z 2024-01-12T06:35:37Z 2023-08-18 2023-06 1976-6696 https://pubs.kist.re.kr/handle/201004/79903 Acute myocardial infarction (AMI) is a multifaceted syndrome influenced by the functions of various extrinsic and intrinsic pathways and pathological processes, which can be detected in circulation using biomarkers. In this study, we investigated the secretome protein profile of induced-hypertrophy cardiomyocytes to identify next-generation biomarkers for AMI diagnosis and management. Hypertrophy was successfully induced in immortalized human cardiomyocytes (T0445) by 200 nM ET-1 and 1 μM Ang II. The protein profiles of hypertrophied cardiomyocyte secretomes were analyzed by nano-liquid chromatography with tandem mass spectrometry and differentially expressed proteins that have been identified by Ingenuity Pathway Analysis. The levels of 32 proteins increased significantly (＞1.4 fold), whereas 17 proteins (＜0.5 fold) showed a rapid decrease in expression. Proteomic analysis showed significant upregulation of six 14-3-3 protein isoforms in hypertrophied cardiomyocytes compared to those in control cells. Multi-reaction monitoring results of human plasma samples showed that 14-3-3 protein-zeta levels were significantly elevated in patients with AMI compared to those of healthy controls. These findings elucidated the role of 14-3-3 protein-zeta in cardiac hypertrophy and cardiovascular disorders and demonstrated its potential as a novel biomarker and therapeutic strategy. English 생화학분자생물학회 Discovery of 14-3-3 zeta as a potential biomarker for cardiac hypertrophy Article 10.5483/BMBRep.2022-0194 1 BMB Reports, v.56, no.6, pp.341 - 346 BMB Reports 56 6 341 346 Y scie scopus kci ART002971396 001079577200004 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article 14-3-3 protein-zeta Acute myocardial infarction Cardiac hypertrophy induced cell Proteomic biomarker