Yu, So Yeon Yoo, Jeong Ha Chun, Myung-Suk Chang, Mi-Sook 2024-01-12T06:55:05Z 2024-01-12T06:55:05Z 2022-03-07 2013-05-14 0000-0000 https://pubs.kist.re.kr/handle/201004/80351 It is very important to separate the human bone marrow-derived mesenchymal stem cells (hMSCs) with high multipotential to differentiation and to increase the efficacy of cell therapy. In this view, we have investigated to develop and validate a novel method for the collection of desired size of hMSCs by utilizing the microfluidic chip with main, side, and branch channels realizing the hydrodynamic filtration (HDF) principle. For the size-dependent cell separation, the PDMS-glass chip was designed to collect the smaller hMSCs at the foremost outlet of the branch channel. The separation performance was controlled by the number of branch channels and the ratio of the flow rate of a main inlet to that of a side inlet. Results of the size distribution of collected cells could be applied for sorting and isolating hMSCs. Further, microfluidic chip filtration has a key advantage capable of continuous operation without applying additional chemicals. English NSTI Size-dependent separation of human mesenchymal stem cells by applying microfluidic chip filtration Conference 1 NSTI-Nanotech 2013, pp.346 - 349 NSTI-Nanotech 2013 346 349 US Washington, DC 2013-05-12 Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo 2-s2.0-84881087084