https://pubs.kist.re.kr/handle/123456789/75369 2026-04-16T13:39:18Z 2026-04-16T13:39:18Z Kim, Tae-Su Yoo, Ji-Hyun Kim, Sang-Yong Pan, Cheol-Ho Kalia, Vipin C. Kang, Yun Chan Lee, Jung-Kul https://pubs.kist.re.kr/handle/201004/125973 2024-04-25T07:07:56Z 2015-01-01T00:00:00Z

Title: Screening and characterization of an Agrobacterium tumefaciens mutant strain producing high level of coenzyme Q(10) Authors: Kim, Tae-Su; Yoo, Ji-Hyun; Kim, Sang-Yong; Pan, Cheol-Ho; Kalia, Vipin C.; Kang, Yun Chan; Lee, Jung-Kul Abstract: A mutant of Agrobacterium tumefaciens (S02-13) producing high levels of coenzyme Q(10) (CoQ(10)), was selected by high-throughput screening after repeated NTG mutagenesis. Mutant S02-13 was resistant to sodium azide and menadione and showed an eight-fold increase in CoQ(10) production, as compared to that of the parent strain. The amount of CoQ(10) produced by this mutant reached 350 mg l(-1) in pH-stat fed-batch culture. Mutant S02-13 differed from wild-type in morphology, biochemical properties, and proteome profiles. Scanning electron microscopy results revealed a remarkable change in morphology: the wild-type strain had a rod shape, whereas the mutant S02-13 was coccoid. In contrast to wild-type, the mutant S02-13 strain showed a negative reaction to tryptophan deaminase and N-2 reduction, and a positive reaction to nitrate production in API kit assays. The spots representing proteins that were increased in the proteome expression of the mutant S02-13 strain were identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH), glucosaminitol dehydrogenase (GlcNOH), and superoxide dismutase (SOD). In particular, the enzyme activities of GAPDH and G1cNOH increased eight-fold and three-fold, respectively, in the mutant, as compared to the wild-type. (C) 2014 Elsevier Ltd. All rights reserved.

2015-01-01T00:00:00Z Kwak, Wonjung Ha, Yeong Su Soni, Nisarg Lee, Woonghee Park, Se-Il Ahn, Heesu An, Gwang Il Kim, In-San Lee, Byung-Heon Yoo, Jeongsoo https://pubs.kist.re.kr/handle/201004/125972 2024-04-25T07:07:55Z 2015-01-01T00:00:00Z

Title: Apoptosis imaging studies in various animal models using radio-iodinated peptide Authors: Kwak, Wonjung; Ha, Yeong Su; Soni, Nisarg; Lee, Woonghee; Park, Se-Il; Ahn, Heesu; An, Gwang Il; Kim, In-San; Lee, Byung-Heon; Yoo, Jeongsoo Abstract: Apoptosis has a role in many medical disorders and treatments; hence, its non-invasive evaluation is one of the most riveting research topics. Currently annexin V is used as gold standard for imaging apoptosis. However, several drawbacks, including high background, slow body clearance, make it a suboptimum marker for apoptosis imaging. In this study, we radiolabeled the recently identified histone H1 targeting peptide (ApoPep-1) and evaluated its potential as a new apoptosis imaging agent in various animal models. ApoPep-1 (CQRPPR) was synthesized, and an extra tyrosine residue was added to its N-terminal end for radiolabeling. This peptide was radiolabeled with I-124 and I-131 and was tested for its serum stability. Surgery- and drug-induced apoptotic rat models were prepared for apoptosis evaluation, and PET imaging was performed. Doxorubicin was used for xenograft tumor treatment in mice, and the induced apoptosis was studied. Tumor metabolism and proliferation were assessed by [F-18]FDG and [F-18]FLT PET imaging and compared with ApoPep-1 after doxorubicin treatment. The peptide was radiolabeled at high purity, and it showed reasonably good stability in serum. Cell death was easily imaged by radiolabeled ApoPep-1 in an ischemia surgery model. And, liver apoptosis was more clearly identified by ApoPep-1 rather than [I-124]annexin V in cycloheximide-treated models. Three doxorubicin doses inhibited tumor growth, which was evaluated by 30-40 % decreases of [F-18]FDG and [F-18]FLT PET uptake in the tumor area. However, ApoPep-1 demonstrated more than 200 % increase in tumor uptake after chemotherapy, while annexin V did not show any meaningful uptake in the tumor compared with the background. Biodistribution data were also in good agreement with the microPET imaging results. All of the experimental data clearly demonstrated high potential of the radiolabeled ApoPep-1 for in vivo apoptosis imaging.

2015-01-01T00:00:00Z Kim, Bo-Yun Seo, In-Tae Lee, Youn-Seon Kim, Jin-Seong Nahm, Sahn Kang, Chong-Yun Yoon, Seok-Jin Paik, Jong-Hoo Jeong, Young-Hun https://pubs.kist.re.kr/handle/201004/125971 2024-04-25T07:07:48Z 2015-01-01T00:00:00Z

Title: High-Performance (Na0.5K0.5)NbO3 Thin Film Piezoelectric Energy Harvester Authors: Kim, Bo-Yun; Seo, In-Tae; Lee, Youn-Seon; Kim, Jin-Seong; Nahm, Sahn; Kang, Chong-Yun; Yoon, Seok-Jin; Paik, Jong-Hoo; Jeong, Young-Hun Abstract: The pseudocubic structure of a (Na0.5K0.5)NbO3 (NKN) film grown on a Pt/Ti/SiO2/Si substrate changed to an orthorhombic structure when the film was transferred onto a polyimide substrate. Piezoelectric constant for the transferred NKN film increased considerably from 74 +/- 11 to 120 +/- 18pm/V because the crystal structure of the film had changed from pseudocubic to orthorhombic. A gold interdigitated electrode was deposited onto the transferred NKN film to synthesize a NKN piezoelectric energy harvester. The NKN piezoelectric energy harvester was poled before bending under a 100kV/cm DC electric field across the electrodes. When a strain of 0.85% and a strain rate of 4.05%/s were applied to the NKN piezoelectric energy harvester, it produced a maximum output voltage of 1.9V and a current of 38nA, corresponding to a power density of 2.89W/cm(3).

2015-01-01T00:00:00Z Lee, Sung-In Jo, Seong-Mu Joh, Han-Ik Lee, Myong-Hoon Lee, Sungho https://pubs.kist.re.kr/handle/201004/125970 2024-04-25T07:07:35Z 2015-01-01T00:00:00Z

Title: Oxygen adsorption-induced surface segregation of titanium oxide by activation in carbon nanofibers for maximizing photocatalytic performance Authors: Lee, Sung-In; Jo, Seong-Mu; Joh, Han-Ik; Lee, Myong-Hoon; Lee, Sungho Abstract: This research demonstrates a simple method for synthesizing titanium dioxide nanoparticle-decorated carbon nanofibers. These nanofibers showed highly efficient degradation of methylene blue under UV light because of the synergistic effects of the large surface-active sites of titanium dioxide nanoparticles and the carbon nanofibers on the photocatalytic properties.

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