https://pubs.kist.re.kr/handle/123456789/75374 2026-04-15T18:20:36Z https://pubs.kist.re.kr/handle/201004/154346 Title: Activity-Based Protein Profiling Reveals Potential Dasatinib Targets in Gastric Cancer Authors: Choi, Kyoung-Min; Cho, Eunji; Bang, Geul; Lee, Seong-Jae; Kim, Boram; Kim, Ji-Hee; Park, Seo-Gyu; Han, Eun Hee; Chung, Young-Ho; Kim, Jin Young; Kim, Eunjung; Kim, Jae-Young Abstract: Dasatinib is a multi-target kinase inhibitor, whose targets include BCR-ABL, SRC family kinases, and various cancer kinases. The elevated SRC activity in gastric cancer (GC) has prompted the need for the therapeutic application of dasatinib in GC. We observed that the efficacy of dasatinib varied with the GC cell lines. The differential effect of dasatinib was not correlated with the basal SRC activity of each cell line. Moreover, the GC cell lines showing the strong antitumor effects of dasatinib were refractory to other SRC inhibitors, i.e., bosutinib and saracatinib, suggesting that unexpected dasatinib’s targets could exist. To profile the targets of dasatinib in GC, we performed activity-based protein profiling (ABPP) via mass spectrometry using a desthiobiotin-ATP probe. We identified 29 and 18 kinases as potential targets in dasatinib-sensitive (SNU-216, MKN-1) and -resistant (SNU-484, SNU-601) cell lines, respectively. The protein–protein interaction mapping of the differential drug targets in dasatinib-sensitive and -resistant GC using the STRING database suggested that dasatinib could target cellular energy homeostasis in the drug-sensitive GC. RNAi screening for identified targets indicated p90RSK could be a novel dasatinib target, which is important for maintaining the viability and motility of GC cells. Further functional validation of dasatinib off-target actions will provide more effective therapeutic options for GC. 2020-12-01T00:00:00Z https://pubs.kist.re.kr/handle/201004/154065 Title: 실시간질량분석장치 (SIFT-MS) 장착 이동측정시스템에 의한 산업단지 유해대기오염물질 오염도 측정 연구 Authors: 윤승진; 조강희; 김형석; 송건범; 이승복; 정주영 Abstract: In this paper, we describe the application of a mobile measurement system with SIFT-MS for measuring gaseous hazardous air pollutants (HAPs) concentrations in the ambient air of an industrial complex. Both stationary and mobile monitoring methods were tested to evaluate the HAPs. The field monitoring campaign was conducted for 7 days in the selected industrial complex, where petroleum manufacturing facilities were located. It was found that the accuracy of SIFT-MS quantification was improved by correcting the reaction rate constant (k-factor) through standard gas testing. In stationary monitoring, methanol showed the highest concentrations regardless of the measuring sites. It is notable that the concentration of some pollutants such as methanol and C2-benzene increased considerably to several hundred ppbv at a certain time, indicating that people living in the industrial complex may be exposed to a high health risk. It was possible to estimate the emission source by analyzing real-time monitoring results and wind direction and speed. Methanol showed the highest concentrations also in the mobile measurements, indicating that methanol is widely emitted in this area. The mobile measurements together with HAPs pollution mapping were used to investigate the spatial distribution of a number of pollutants concentrations. We believe that this measuremet system using SIFT-MS is a useful tool for evaluating the temporal and spatial concentrations of HAPs in the ambient air of the vicinity of industrial complexes instead of conventional analysis techniques such as gas chromatography. 2020-08-01T00:00:00Z https://pubs.kist.re.kr/handle/201004/154064 Title: 도시철도 터널과 대합실에서 미세먼지 농도의 일변화 특징 Authors: 김종범; 이승복; 우상희; 김창혁; 이선엽; 이재인; 배귀남 Abstract: Since 1974, the urban subway has been used as a major form of public transportation in Seoul, Korea. The air quality in the subway environment depends on the introduction of air pollutants from roadway air and its generation is caused by subway operation in the tunnel. In the subway tunnel, PM10 concentration was monitored from March 8 to 15, 2018 and from March 26 to 28, 2018, and compared with concentrations that are routinely monitored at the subway concourse and the nearest roadside air quality monitoring station (RAQMS). Overall PM10 concentration at the concourse was similar to that of the RAQMS. However, PM10 concentration in the tunnel was significantly higher than those of the subway concourse and RAQMS, and showed distinct diurnal variation caused by train operation. The dominant peak concentrations were highly correlated with the number of train operations per hour. The minimum PM10 concentration was observed between 2 am to 5 am when the train was not operated. This was similar to that of the RAQMS. Although the diurnal variation of the PM10 concentration at the concourse is not significant, the overall trend is similar to that in the tunnel. 2020-09-01T00:00:00Z https://pubs.kist.re.kr/handle/201004/119179 Title: Attenuation of STAT3 Signaling Cascade by Daidzin Can Enhance the Apoptotic Potential of Bortezomib against Multiple Myeloma Authors: Yang, Min Hee; Jung, Sang Hoon; Chinnathambi, Arunachalam; Alahmadi, Tahani Awad; Alharbi, Sulaiman Ali; Sethi, Gautam; Ahn, Kwang Seok Abstract: Daidzin (DDZ) extracted from Pueraria lobate (Fabaceae) is a widely known phytoestrogen. DDZ can display anti-cancer activities against breast and prostate cancers, but its anti-oncogenic actions in multiple myeloma (MM) cells have not been studied. The signal transducer and activator of transcription 3 (STAT3) can control key processes including proliferation, differentiation, and survival in MM cells. Here, we noted that DDZ abrogated STAT3 activation (both constitutive as well as inducible) at Tyr705 and Ser727 in MM cells. Additionally, DDZ mitigated the phosphorylation of STAT3 upstream Janus-activated kinases (JAK1/2) and c-Src kinases. Pervanadate (tyrosine phosphatase blocker) exposure altered the DDZ-induced inhibition of STAT3 activation, thus affecting the action of this phytoestrogen on apoptosis. Moreover, DDZ impeded proliferation and augmented the apoptotic effects of bortezomib (Bor) in MM cells. Overall, the data indicate that DDZ may act as a potent suppressor of STAT3 signaling cascade, and the co-treatment of DDZ and Bor could be a promising therapeutic strategy, specifically in MM. 2020-01-01T00:00:00Z