Full metadata record

DC Field Value Language
dc.contributor.authorKim, H.J.-
dc.contributor.authorChoi, W.-
dc.contributor.authorSan, Lee J.-
dc.contributor.authorChoi, J.-
dc.contributor.authorChoi, N.-
dc.contributor.authorHwang, K.S.-
dc.date.accessioned2024-01-19T13:02:46Z-
dc.date.available2024-01-19T13:02:46Z-
dc.date.created2021-10-21-
dc.date.issued2022-01-
dc.identifier.issn0956-5663-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/115916-
dc.description.abstractAnalysis of a ratio between amyloid beta 1?40 and 1?42 (Aβ1-40 and Aβ1-42) presented in plasm enables a highly accurate diagnosis of Alzheimer's disease (AD). However, the analysis of plasma Aβs is not routinely conducted because of the lack of Aβ detection techniques sensitive enough to specifically detect Aβ from thousands of biomaterials present in the plasma. We developed a hydrogel-patterned spiral microelectrode sensor combined with a hopping dielectrophoretic (DEP) force, combining the negative DEP and positive DEP forces, for Aβ detection. The hydrogel effectively increased the number of immobilized fragmented antibodies in the reaction region of the sensor and enabled size-exclusive passive filtration of non-specific plasma proteins from that region. The hopping DEP force further concentrated the Aβs and removed the non-specific plasma proteins. Consequently, our sensor achieved a limit of detection (LOD) of approximately ∼ 0.15 pg/mL for both Aβ1-40 and Aβ1-42 in the standard plasma. Finally, comparing the ratio between Aβ1-40 and Aβ1-42 signals, we distinguished AD patients from cognitively normal subjects with 95.83% accuracy and 92.31% precision (n = 24, p < 0.0001, One-way ANOVA). ? 2021 The Authors-
dc.languageEnglish-
dc.publisherPergamon Press Ltd.-
dc.titleClinical application of serological Alzheimer's disease diagnosis using a highly sensitive biosensor with hydrogel-enhanced dielectrophoretic force-
dc.typeArticle-
dc.identifier.doi10.1016/j.bios.2021.113668-
dc.description.journalClass1-
dc.identifier.bibliographicCitationBiosensors and Bioelectronics, v.195-
dc.citation.titleBiosensors and Bioelectronics-
dc.citation.volume195-
dc.description.isOpenAccessY-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.identifier.wosid000705223300005-
dc.identifier.scopusid2-s2.0-85115817184-
dc.relation.journalWebOfScienceCategoryBiophysics-
dc.relation.journalWebOfScienceCategoryBiotechnology & Applied Microbiology-
dc.relation.journalWebOfScienceCategoryChemistry, Analytical-
dc.relation.journalWebOfScienceCategoryElectrochemistry-
dc.relation.journalWebOfScienceCategoryNanoscience & Nanotechnology-
dc.relation.journalResearchAreaBiophysics-
dc.relation.journalResearchAreaBiotechnology & Applied Microbiology-
dc.relation.journalResearchAreaChemistry-
dc.relation.journalResearchAreaElectrochemistry-
dc.relation.journalResearchAreaScience & Technology - Other Topics-
dc.type.docTypeArticle-
dc.subject.keywordPlusDielectric devices-
dc.subject.keywordPlusGlycoproteins-
dc.subject.keywordPlusHydrogels-
dc.subject.keywordPlusMicroelectrodes-
dc.subject.keywordPlusNeurodegenerative diseases-
dc.subject.keywordPlusAlzheimers disease-
dc.subject.keywordPlusAmyloid betas-
dc.subject.keywordPlusClinical application-
dc.subject.keywordPlusDielectrophoretic forces-
dc.subject.keywordPlusDisease diagnosis-
dc.subject.keywordPlusHopping dielectrophoretic force-
dc.subject.keywordPlusPlasma protein-
dc.subject.keywordPlusPlasma-based diagnose-
dc.subject.keywordPlusPorosity-tunable hydrogel-
dc.subject.keywordPlusTunables-
dc.subject.keywordPlusDiagnosis-
dc.subject.keywordAuthorAlzheimer&apos-
dc.subject.keywordAuthors disease-
dc.subject.keywordAuthorAmyloid-beta-
dc.subject.keywordAuthorHopping dielectrophoretic force-
dc.subject.keywordAuthorPlasma-based diagnosis-
dc.subject.keywordAuthorPorosity-tunable hydrogel-
Appears in Collections:
KIST Article > 2022
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE