Photoacoustic imaging of cancer cells with glycol-chitosan-coated gold nanoparticles as contrast agents

Authors
Sun, In-CheolAhn, Cheol-HeeKim, KwangmeyungEmelianov, Stanislav
Issue Date
2019-12
Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
Citation
JOURNAL OF BIOMEDICAL OPTICS, v.24, no.12
Abstract
Utility of glycol-chitosan-coated gold nanoparticles (GC-AuNPs) as a photoacoustic contrast agent for cancer cell imaging was demonstrated. Through the synergistic effect of glycol chitosan and gold nanoparticles, GC-AuNPs showed cellular uptake in breast cancer cells and resulted in strong photoacoustic signals in tissue-mimicking cell phantoms. The performance of GC-AuNPs as contrast agents was established with photoacoustic imaging and confirmed with dark-field microscopy. The cell phantoms displayed strong photoacoustic signals if cells were incubated more than 3 h with GC-AuNPs, compared with PEG-AuNPs that showed no photoacoustic signal increase. The enhanced photoacoustic signals originated from the plasmon coupling effect of GC-AuNPs after the cellular uptake in cancer cells. Importantly, photoacoustic imaging of cancer cells was achieved with GC-AuNPs-contrast agents that did not require antibodies or complex surface modification. The endocytosis of GC-AuNPs was also confirmed with dark-field microscopy. The results show that GC-AuNPs have potential as a photoacoustic contrast agent for cellular imaging including tumor tissue imaging. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License.
Keywords
ULTRASOUND; CARRIERS; ULTRASOUND; CARRIERS; glycol chitosan; gold nanoparticles; photoacoustic imaging; cancer imaging; contrast agent
ISSN
1083-3668
URI
https://pubs.kist.re.kr/handle/201004/119276
DOI
10.1117/1.JBO.24.12.121903
Appears in Collections:
KIST Article > 2019
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE