Chemical Constituents from Leaves of Hydrangea serrata and Their Anti-photoaging Effects on UVB-Irradiated Human Fibroblasts

Authors
Shin, Ji-SunHan, Hee-SooLee, Seung-BinMyung, Da-binLee, KeunsukLee, Sun HeeKim, Hyoung JaLee, Kyung-Tae
Issue Date
2019-03
Publisher
PHARMACEUTICAL SOC JAPAN
Citation
BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.42, no.3, pp.424 - 431
Abstract
Hydrangea serrata (THUMB.) SER. (Hydrangeaceae) leaves have been used as herbal teas in Korea and Japan. The objective of this study was to identify anti-photoaging compounds in aqueous EtOH extract prepared from leaves of H. serrata and their effects on UVB-irradiated Hs68 human foreskin fibroblasts. Phytochemical study on H. serrata leaves led to the isolation and characterization of ten compounds: hydrangenol, thunberginol A, thunberginol C, hydrangenoside A, hydrangenoside C, cudrabibenzyl A, 2,3,4'-trihydroxystilbene, thunberginol F, quercetin 3-O-beta-D-xylopyranosyl (1-2)-beta-D-galactopyranoside, quercetin 3-O-beta-D-xylopyranosyl (1-2)-beta-D-glucopyranoside. Cudrabibenzyl A, 2,3,4'-trihydroxystilbene, quercetin 3-O-beta-D-xylopyranosyl (1-2)-beta-D-galactopyranoside, quercetin 3-O-beta-D-xylopyranosyl (1-2)-beta-D-glucopyranoside were firstly isolated from H. serrata. We estimated the effects of 10 compounds on cell viability and production of pro-collagen Type I, matrix metalloproteinase (MMP)-1, and hyaluronic acid (HA) after UVB irradiation. Of these compounds, hydrangenol showed potent preventive activities against reduced cell viability and degradation of pro-collagen Type I in UVB-irradiated Hs68 fibroblasts. Hydrangenol had outstanding inductive activities on HA production. It suppressed mRNA expression levels of MMP-1, MMP-3, hyaluronidase (HYAL)-1, HYAL-2, cyclooxygenase-2 (COX-2), interleukin (IL)-6, IL-8, and IL-1 beta in UVB-irradiated Hs68 fibroblasts. When Hs68 fibroblasts were exposed to hydrangenol after UVB irradiation, UVB-induced reactive oxygen species (ROS) production was suppressed. Hydrangenol also inhibited the activation of activator protein-1 (AP-1) and signal transduction and activation of transcription 1 (STAT-1) by downregulating phosphorylation of p38 and extracellular signal-regulated kinase (ERK). Our data indicate that hydrangenol isolated from H. serrata leaves has potential protective effects on UVB-induced skin photoaging.
Keywords
HYALURONIC-ACID; SKIN; MECHANISMS; PHOSPHORYLATION; SUPPRESSION; GLUCOSIDES; ACTIVATION; EXTRACT; HYALURONIC-ACID; SKIN; MECHANISMS; PHOSPHORYLATION; SUPPRESSION; GLUCOSIDES; ACTIVATION; EXTRACT; Hydrangea serrata; photoaging; matrix metalloproteinase (MMP); procollagen type 1; skin fibroblast
ISSN
0918-6158
URI
https://pubs.kist.re.kr/handle/201004/120289
DOI
10.1248/bpb.b18-00742
Appears in Collections:
KIST Article > 2019
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