Mapping of excitatory and inhibitory postsynaptic potentials of neuronal populations in hippocampal slices using the GEVI, ArcLight

Authors
Nakajima, RyuichiBaker, Bradley J.
Issue Date
2018-12-19
Publisher
IOP PUBLISHING LTD
Citation
JOURNAL OF PHYSICS D-APPLIED PHYSICS, v.51, no.50
Abstract
To understand the circuitry of the brain, it is essential to clarify the functional connectivity among distinct neuronal populations. For this purpose, neuronal activity imaging using genetically-encoded calcium sensors such as GCaMP has been a powerful approach due to its cell-type specificity. However, calcium (Ca2+) is an indirect measure of neuronal activity. A more direct approach would be to use genetically encoded voltage indicators (GEVIs) to observe subthreshold, synaptic activities. The GEVI, ArcLight, which exhibits large fluorescence transients in response to voltage, was expressed in excitatory neurons of the mouse CA1 hippocampus. Fluorescent signals in response to the electrical stimulation of the Schaffer collateral axons were observed in brain slice preparations. ArcLight was able to map both excitatory and inhibitory inputs projected to excitatory neurons. In contrast, the Ca2+ signal detected by GCaMP6f, was only associated with excitatory inputs. ArcLight and similar voltage sensing probes are also becoming powerful paradigms for functional connectivity mapping of brain circuitry.
Keywords
HYBRID VOLTAGE SENSOR; FLUORESCENT PROTEIN; IN-VIVO; SYNAPTIC POTENTIALS; ELECTRICAL-ACTIVITY; PYRAMIDAL NEURONS; BRAIN; CONNECTIVITY; INTERNEURONS; DYNAMICS; HYBRID VOLTAGE SENSOR; FLUORESCENT PROTEIN; IN-VIVO; SYNAPTIC POTENTIALS; ELECTRICAL-ACTIVITY; PYRAMIDAL NEURONS; BRAIN; CONNECTIVITY; INTERNEURONS; DYNAMICS; ArcLight; GCaMP; GEVI; GECI; hippocampus; synaptic excitation; synaptic inhibition
ISSN
0022-3727
URI
https://pubs.kist.re.kr/handle/201004/120563
DOI
10.1088/1361-6463/aae2e3
Appears in Collections:
KIST Article > 2018
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE