THE PROTECTIVE EFFECT OF ANTIOXIDANT ENRICHED FRACTIONS FROM COLORED POTATOES AGAINST HEPATOTOXIC OXIDATIVE STRESS IN CULTURED HEPATOCYTES AND MICE

Authors
Kim, Da HyeKim, MyungsukOh, Sang-BinLee, Kyung MiKim, Sang MinNho, Chu WonYoon, Won ByongKang, KyungsuPan, Cheol-Ho
Issue Date
2017-02
Publisher
WILEY
Citation
JOURNAL OF FOOD BIOCHEMISTRY, v.41, no.1
Abstract
Herein, we prepared standardized antioxidant-enriched fractions from red (AFR) and purple (AFP) colored potatoes and evaluated their protective effects against hepatotoxicity induced by oxidative stress. The major compound contained in both AFR and AFP was 5-caffeoylquinic acid. Experiments in vitro showed that AFR and AFP protected against HepG2 cell death induced by tert-butyl hydroperoxide (t-BHP) as determined by cell viability assay. AFR and AFP recovered alanine aminotransferase (ALT) and aspartate aminotransferase (AST) enzyme activities, which increased by t-BHP treatment in HepG2 cells. AFR and AFP also inhibited the cleavage of PARP and caspase-3 in HepG2 cells. Moreover, in vivo experiments in mice showed that AFR and AFP protected against t-BHP-induced hepatotoxicity and recovered serum ALT and AST enzyme activities. AFR and AFP also rescued serum antioxidant enzyme activities, catalase and glutathione peroxidase, which were disturbed by t-BHP treatment. Our data clearly demonstrate that AFR and AFP protect against hepatic damage induced by oxidative stress.
Keywords
PURPLE POTATO; HYDROXYCINNAMIC ACIDS; ANTHOCYANINS; EXTRACT; DAMAGE; LIVER; APOPTOSIS; PHENOLICS; DIGESTION; CANCER; PURPLE POTATO; HYDROXYCINNAMIC ACIDS; ANTHOCYANINS; EXTRACT; DAMAGE; LIVER; APOPTOSIS; PHENOLICS; DIGESTION; CANCER; colored potato; antioxidant; hepatoprotective; anthocyanin; hydroxycinnamate; HepG2
ISSN
0145-8884
URI
https://pubs.kist.re.kr/handle/201004/123164
DOI
10.1111/jfbc.12315
Appears in Collections:
KIST Article > 2017
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE