Inhibitory effect of the Larix sibirica and its various flavonoids on the IgE-stimulated mast cell activation and anaphylaxis
- Authors
- Kim, Myungsuk; Kim, Sun Young; Randy, Ahmad; Lim, Sue Ji; Dorjsembe, Banzragch; Nho, Chu Won
- Issue Date
- 2016-12
- Publisher
- ELSEVIER SCIENCE BV
- Citation
- JOURNAL OF FUNCTIONAL FOODS, v.27, pp.631 - 644
- Abstract
- Larix sibirica (Siberian larch) is a deciduous tree native to Russia, Mongolia, and China and has been reported to possess anti-viral, anti-oxidant, and neuroprotective properties. In this study, we assessed the anti-allergic effects of an L. sibirica extract (LSE) and its various flavonoids using an IgE-sensitized mast cell-like cell line, rat basophilic leukaemia (RBL)-2H3, and a passive anaphylaxis mouse model. In IgE-sensitized RBL-2H3 cells, LSE and several flavonoids reduced release and production of beta-hexosaminidase, histamine, and inflammatory mediators (prostaglandin D-2, leukotriene C4, interleuldn-4, and tumour necrosis factor-alpha), reduced expression of cyclooxygenase-2 and 5-lipoxygenase, and suppressed activation of nuclear factor-kappa B. LSE and several flavonoids reduced phosphorylation of downstream signalling molecules, such as Syk, protein kinase C mu, phospholipase C gamma, and mitogen-activated protein kinases. Oral administration of LSE inhibited mast cell-dependent passive cutaneous anaphylactic reactions. These results suggest that LSE is a beneficial candidate for the prevention and treatment of allergic disorders. (C) 2016 Elsevier Ltd. All rights reserved.
- Keywords
- PASSIVE CUTANEOUS ANAPHYLAXIS; RBL-2H3 CELLS; TNF-ALPHA; STRUCTURAL REQUIREMENTS; SIGNALING PATHWAYS; PHENOLIC-COMPOUNDS; LEUKOTRIENE C-4; SIBERIAN LARCH; KAPPA-B; DEGRANULATION; PASSIVE CUTANEOUS ANAPHYLAXIS; RBL-2H3 CELLS; TNF-ALPHA; STRUCTURAL REQUIREMENTS; SIGNALING PATHWAYS; PHENOLIC-COMPOUNDS; LEUKOTRIENE C-4; SIBERIAN LARCH; KAPPA-B; DEGRANULATION; Larix sibirica; Flavonoids; Mast cell RBL-2H3; IgE; Fc epsilon RI signalling
- ISSN
- 1756-4646
- URI
- https://pubs.kist.re.kr/handle/201004/123394
- DOI
- 10.1016/j.jff.2016.10.009
- Appears in Collections:
- KIST Article > 2016
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