Burkholderia jirisanensis sp nov., isolated from forest soil

Abstract

A Gram-negative, catalase-positive, mesophilic, obligately aerobic bacterium designated JRM2-1(T) was isolated from forest soil of Jirisan Mountain, Republic of Korea, and its taxonomic position was investigated based on a polyphasic taxonomic approach. Cells of strain JRM2-1(T) grew optimally at pH 5.0-7.0 and at 25 degrees C. Strain JRM2-1(T) was susceptible to chloramphenicol, gentamicin, kanamycin, nalidixic acid, rifampicin, streptomycin and tetracycline. On the basis of 16S rRNA gene sequence similarity, the closest neighbour of strain JRM2-1(T) was Burkholderia rhizosphaerae WR43(T) (98.1 %). On the basis of our phylogenetic analysis, strain JRM2-1(T) is clearly distinguished from related species of the genus Burkholderia and is clustered with plant-associated members of the genus. The major cellular fatty acids were C-16 : 0, C-17 : 0 cyclo and C-19 : 0 cyclo omega 8c. The polar lipid profile of strain JRM2-1(T) contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, several unidentified aminolipids and an unidentified aminophospholipid. The isoprenoid quinone of strain JRM2-1(T) was Q-8 and the DNA G + C content was 63.7 mol%. On the basis of our polyphasic taxonomic investigation, strain JRM2-1(T) is considered to represent a novel species in the genus Burkholderia, for which the name Burkholderia jirisanensis sp. nov. is proposed. The type strain is JRM2-1(T) (=AIM 0373(T) =KCTC 42072(T) =JCM 19985(T)).