Hydrophillic Interaction Chromatography-tandem Mass Spectrometry Method for Identification and Quantitation of 5-MeO-DIPT and its Metabolites in Rat Urine

Authors
Kim, YoonKim, Un YongIn, Moon KyoLee, JaeickKwon, Oh-SeungYoo, Hye Hyun
Issue Date
2011-04
Publisher
대한화학회
Citation
Bulletin of the Korean Chemical Society, v.32, no.4, pp.1158 - 1164
Abstract
5-Methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT), a psychoactive tryptamine derivative, is a hallucinogenic drug of abuse. In this study, 5-OH-DIPT and its metabolites were identified and the quantitative method was developed and validated by using hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-MS/MS). Chromatographic separation was achieved on an Atlantis HILIC silica column (5 mu m, 100 x 2.1 mm). The metabolites of 5-MeO-DIPT in rat urine were characterized via Q1 scanning and product ion scanning. As a consequence, 5-MeO-IPT, 5-OH-DIPT, 6-OH-5-MeO-DIPT and their glucuronide conjugates were detected and identified as the metabolites of 5-MeO-DIPT. Subsequently, a quantitative method for 5-MeO-DIPT and its major metabolites, 5-MeO-IPT and 5-OH-DIPT, was developed in multiple reactions monitoring (MRM) mode. The calibration curves for all analytes evidenced good linearity over the concentration range of 11000 ng/mL with linear correlation co-efficients (r(2)) in excess of 0.99. The intra- and inter-day accuracy and precision were 92.2-110.2% and 1.5-9.9%, respectively.
Keywords
5-METHOXY-N,N-DIISOPROPYLTRYPTAMINE; PHENETHYLAMINES; CONFIRMATION; TRYPTAMINES; FOXY; 5-MeO-DIPT; HILIC-MS/MS; Metabolites; Profiling; Quantitation
ISSN
0253-2964
URI
https://pubs.kist.re.kr/handle/201004/130478
DOI
10.5012/bkcs.2011.32.4.1158
Appears in Collections:
KIST Article > 2011
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