Evaluation of the Antioxidant Activity of the Fruiting Body of Phellinus linteus Using the On-line HPLC-DPPH Method

Authors
Jeon, Young EunLee, Yeon SilLim, Soon SungKim, Sun-JuJung, Sang HoonBae, Young-SooYi, Jae-SeonKang, Il-Jun
Issue Date
2009-10
Publisher
KOREAN SOC APPLIED BIOLOGICAL CHEMISTRY
Citation
JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY, v.52, no.5, pp.472 - 479
Abstract
Phellinus linteus has traditionally been used as a medicinal mushroom in Oriental countries. Bioactive phytochemicals from the fruiting body of P. linteus were investigated using the on-line HPLC-DPPH system. The system was used to separate 10 antioxidants: protocatechuic acid (1) protocatechualdehyde, (2) caffeic acid (3), ellagic acid (4), hispidin (5), davalliallactone (6), interfungins A (7), hypholomine B (8), inoscavin A (9), and methyldavallialactone (10). Of these, compounds 6 and 7 showed the strongest inhibitory effect against the DPPH radical and superoxide anion radical-scavenging capacity. On comparison with quercetin (IC50 44.0 mu M), the IC50 value of compounds 6 and 7 for DPPH radical-scavenging capacity were 19.6 mu M and 18.5 mu M. Moreover, the antioxidative capacity of compound 6 at 40 mM was 2.95 nmol Trolox equivalents per mg dry wt. These results indicated that hispidin dimers such as hispidin with hispidin or hispolon had a stronger effect on radical-scavenging capacity than hispidin itself.
Keywords
RADICAL SCAVENGING COMPOUNDS; ALDOSE REDUCTASE; HISPIDIN; L.; CONSTITUENTS; INHIBITOR; CELLS; RADICAL SCAVENGING COMPOUNDS; ALDOSE REDUCTASE; HISPIDIN; L.; CONSTITUENTS; INHIBITOR; CELLS; anti-oxidants; DPPH radicals; on-line RP-HPLC-DPPH; Phellinus linteus; superoxide anion radical-scavenging capacity
ISSN
1738-2203
URI
https://pubs.kist.re.kr/handle/201004/132101
DOI
10.3839/jksabc.2009.081
Appears in Collections:
KIST Article > 2009
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