Multi-well chip for forming a uniform embryoid body in a tiny droplet with mouse embryonic stem cells
- Authors
- Kim, Choong; Lee, In Hye; Lee, Kangsun; Ryu, Sung Shin; Lee, Sang Ho; Lee, Kyu-Jung; Lee, Jinwoo; Kang, Ji Yoon; Kim, Tae Song
- Issue Date
- 2007-12
- Publisher
- TAYLOR & FRANCIS LTD
- Citation
- BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.71, no.12, pp.2985 - 2991
- Abstract
- A multi-well chip (MWC) is described by which mouse embryonic carcinoma (EC) stem cells form a comparatively more rapid and uniform embryoid body (EB) over the conventional hanging drop (HD) method. The newly developed MWC consists of an array of extruded through-holes, each of which holds a droplet of the cell suspension. The study found that the small curvature radius of the droplet in the MWC improved the EB formation rate of a hanging drop from 70% to 98%. Furthermore, the EBs formed by the MWC were uniformly round in shape regardless of the number of suspended cells ranging from 0.5 x 10(3) to 20 x 10(3). The ratio of beating colonies from the MWC was over 2-fold larger than that from HD. The experiments demonstrate that the MWC will be a valuable experimental tool for robust and reproducible EB-based differentiation of a defined number of ES cells.
- Keywords
- IN-VITRO; DIFFERENTIATION; CARDIOMYOCYTES; EFFICIENCY; CULTURE; BODIES; IN-VITRO; DIFFERENTIATION; CARDIOMYOCYTES; EFFICIENCY; CULTURE; BODIES; embryoid body; multi-well chip; hanging drop; differentiation
- ISSN
- 0916-8451
- URI
- https://pubs.kist.re.kr/handle/201004/133944
- DOI
- 10.1271/bbb.70384
- Appears in Collections:
- KIST Article > 2007
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