Reversine stimulates adipocyte differentiation and downregulates Akt and p70(s6k) signaling pathways in 3T3-L1 cells

Authors
Kim, Yong KeeChoi, Hye-YoungKim, Nam HyunLee, WoojungSeo, Dong-WanKang, Dong-WonLee, Hoi YoungHan, Jeung-WhanPark, Sahng WookKim, Su-Nam
Issue Date
2007-06-29
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.358, no.2, pp.553 - 558
Abstract
In this study, we investigate the ability of reversine to stimulate adipocyte differentiation and its effect on cellular signaling pathways associated with adipocyte differentiation. Our data show that reversine treatment of 3T3-L1 cells under differentiation conditions synergistically enhances adipocyte differentiation and the expression of adipogenic marker genes such as aP2, PPAR-gamma, resistin, C/EBP alpha, and adiponectin. In parallel, reversine treatment leads to a selective downregulation of Akt and p70(s6k) signaling pathways, but not the ERK pathway. Furthermore, reversine stimulation of adipocyte differentiation seems to be quite different from troglitazone's action, because reversine treatment does not induce the transcriptional activation of PPAR-gamma and troglitazone does not affect the Akt and p70(s6k) signaling pathways. Taken together, our data clearly demonstrate the ability of reversine to stimulate adipocyte differentiation, which is independent of the Akt and p70(s6k) signaling pathways. (C) 2007 Elsevier Inc. All rights reserved.
Keywords
ACTIVATED RECEPTOR-GAMMA; INSULIN-RECEPTOR; SKELETAL-MUSCLE; GLUCOSE-UPTAKE; PPAR-GAMMA; IN-VITRO; ADIPOGENESIS; RAPAMYCIN; ALPHA; KINASE; ACTIVATED RECEPTOR-GAMMA; INSULIN-RECEPTOR; SKELETAL-MUSCLE; GLUCOSE-UPTAKE; PPAR-GAMMA; IN-VITRO; ADIPOGENESIS; RAPAMYCIN; ALPHA; KINASE; reversine; adipocyte differentiation; Akt; p70(s6k)
ISSN
0006-291X
URI
https://pubs.kist.re.kr/handle/201004/134311
DOI
10.1016/j.bbrc.2007.04.165
Appears in Collections:
KIST Article > 2007
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