A three-dimensional model of fluid-structural interactions for quantifying the contractile force for cardiomyocytes on hybrid biopolymer microcantilever

Abstract

Quantitatively analysis of the contractility of cardiomyocytes is important for understanding the, mechanism of heart failure as well as the molecular alterations in diseased heart cells. This paper presents a realistic computational model, which considers the three-dimensional fluid-structural interactions (FSI), to quantify the contractile force of cardiomyocytes on hybrid biopolymer microcantilevers. Prior to this study, only static modeling of the microscale cellular force has been reported. This study modeled the dynamics of cardiomyocytes on microcantilevers in a medium using the FSL This realistic model was compared with static finite element modeling (FEM) analysis and the Stoney's equation-based analytical solution, and was validated by the deflections of the microcanti levers in the experimental results. Using harmonic response analysis in FSI modeling, the motion of a hybrid biopolymer microcantilever in the medium was identified as a second-order system and the influence of the dynamics of cardiornyocytes could be evaluated quantitatively. (c) 2007 Elsevier Ltd. All rights reserved.