Prenatal GABA(B1) and GABA(B2) receptors: Cellular and subcellular organelle localization in early fetal rat cortical neurons

Authors
Li, S. P.Lee, H. Y.Park, M. S.Bahk, J. Y.Chung, B. C.Kim, M. O.
Issue Date
2006-12-15
Publisher
WILEY
Citation
SYNAPSE, v.60, no.8, pp.557 - 566
Abstract
gamma-aminobutyric acid (GABA)(B) receptors appear to influence developmental events, depending on whether they are found at a synapse or in extrasynaptic areas. Little, if anything, is known as to the cellular and subcellular localization of GABA(B1), and GABA(B2) receptors during early fetal development. We used Western blots, immunohistochemistry, and postembedding immunoelectronmicroscopy to investigate fetal rat brain expression and distribution of these receptor proteins. GABA(B1), is expressed as early as gestational day (GD) 11.5 and 12.5, with immunoreactivity found in the all neuroepithelium, and a high expression in the mantel zone and the cortical area's plate; no immunolabeling for GABA(B2) receptor was observed. Our immunogold studies define a pattern of early GABA(B1) receptor protein in dendrite processes, endoplasmic reticulum, and axon terminals of the cortical neuroepithelium. on GD 11.5. On GD 12.5, GABA(B1), receptor immunogold was found in dendrite processes, spines and tree, axon terminals, mitochrondria, and intracellular organelles of the cortical neuroepithelium. No synapse formation was apparent as no synaptophysin could be found on either GD 11.5 or 12.5. We suggest that GABA(B1), has a functional role in the early fetal brain during neuronal proliferation and migration, and that it is different from the established functional GABA(B) receptor.
Keywords
SUBUNIT MESSENGER-RNAS; TRANSIENT EXPRESSION; TANGENTIAL MIGRATION; NEURITE OUTGROWTH; CELLS; NEOCORTEX; CHEMOKINESIS; CHEMOTAXIS; PATTERNS; ONTOGENY; SUBUNIT MESSENGER-RNAS; TRANSIENT EXPRESSION; TANGENTIAL MIGRATION; NEURITE OUTGROWTH; CELLS; NEOCORTEX; CHEMOKINESIS; CHEMOTAXIS; PATTERNS; ONTOGENY; GABA(B) receptors; early fetal rat brain; cortex; cellular and subcellular localization; immunogold
ISSN
0887-4476
URI
https://pubs.kist.re.kr/handle/201004/134814
DOI
10.1002/syn.20332
Appears in Collections:
KIST Article > 2006
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE