Label-free protein assay with site-directly immobilized antibody using self-actuating PZT cantilever

Authors
Kang, Ghi YuunHan, Ga YoungKang, Ji YoonCho, Il-HoonPark, Hyung-HoPaek, Se-HwanKim, Tae Song
Issue Date
2006-10-12
Publisher
ELSEVIER SCIENCE SA
Citation
SENSORS AND ACTUATORS B-CHEMICAL, v.117, no.2, pp.332 - 338
Abstract
This paper reports a method to improve the limit of the label-free detection of myoglobin using site-directed immobilization on the surface of self-actuating PZT cantilever sensor. The resonant frequency change of cantilever was measured electrically using piezoelectricity when antibody-antigen interaction occurred. Site-directed antibody was biotinylated after reducing the binding region to hinge region of randomly biotinylated antibody, which increased the number of effective binding sites. Investigating the effect of site-directed antibody on the cantilever sensor revealed that this protocol was able to enhance the performance of analytical sensitivity about 10 times with the modification of sensing layer. The experimental results demonstrated this biosensor can be used for diagnosis of acute myocardial infarction in clinical range of tens of ng/ml. (c) 2005 Elsevier B.V. All rights reserved.
Keywords
ELECTRICAL DETECTION; CARDIAC MARKERS; MICROCANTILEVER; POINT; ELECTRICAL DETECTION; CARDIAC MARKERS; MICROCANTILEVER; POINT; PZT microcantilever; electrical detection; site-directly immobilized antibody; myoglobin
ISSN
0925-4005
URI
https://pubs.kist.re.kr/handle/201004/135029
DOI
10.1016/j.snb.2005.11.011
Appears in Collections:
KIST Article > 2006
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