Suppressive effect of inducible nitric oxide synthase (iNOS) expression by the methanol extract of Actinodaphne lancifolia

Authors
Kim, YMin, HYPark, HJLee, EJPark, EJHwang, HJJin, CLee, YSLee, SK
Issue Date
2004-10
Publisher
WILEY
Citation
PHYTOTHERAPY RESEARCH, v.18, no.10, pp.853 - 856
Abstract
Nitric oxide (NO) produced by inducible nitric oxide synthase (iNOS) has played a crucial role in various pathophysiological processes including inflammation and carcinogenesis. Therefore, the inhibitors of NO synthesis or iNOS gene expression have been considered as potential anti-inflammatory and cancer chemopreventive agents. In our continuous search for iNOS inhibitors from natural products we have evaluated indigenous Korean plant extracts using an assay for inhibition of nitric oxide formation on lipopolysaccharide (LPS)activated mouse macrophage RAW 264.7 cells. As a result, the methanolic stem extract of Actinodaphne lancifolia showed an inhibitory activity of NO production in a dose-dependent manner (IC50 = 2.5 mug/ml). Additional study demonstrated that the extract of Actinodaphne lancifolia significantly suppressed the iNOS protein and gene expression in a dose-dependent manner. These results suggest that Actinodaphne lancifolia could be a potential candidate for developing an iNOS inhibitor from natural products. Further elucidation of active principles for development of new cancer chemopreventive and/or anti-inflammatory agents could be warranted. Copyright (C) 2004 John Wiley Sons, Ltd.
Keywords
CANCER; CARCINOGENESIS; MACROPHAGES; PROGRESSION; INHIBITION; GENE; HEAD; NECK; CANCER; CARCINOGENESIS; MACROPHAGES; PROGRESSION; INHIBITION; GENE; HEAD; NECK; inducible nitric oxide synthase (NOS); Actinodaphne lancifolia; lipopolysaccharide (LPS); nitric oxides RAW 264.7 cells
ISSN
0951-418X
URI
https://pubs.kist.re.kr/handle/201004/137215
DOI
10.1002/ptr.1558
Appears in Collections:
KIST Article > 2004
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE