Elevated urinary 8-isoprostaglandin F-2 alpha in females with Graves' hyperthyroidism

Abstract

In this study, we investigate how oxidative stress alters the urinary F-2-isoprostanes&apos; level and we examine the correlation between 8-isoPGF(2alpha) and thyroid hormones in female patients with Graves&apos; hyperthyroidism. We quantitatively determined the concentrations of urinary F-2-isoprostanes using gas chromatography-mass spectrometry in the selected ion-monitoring mode. We recruited individuals in the following three groups of subjects for this study: (1) female hyperthyroidism patients (n = 14, 21-71 years), (2) female hypothyroidism patients (n = 16, 16-66 years), and (3) female age-matched normal controls (n = 10, 20-61 years). The average concentration of 8-iso prostaglandin F-2alpha (8-isoPGF(2alpha)) in hyperthyroidism patients was significantly higher than that in the healthy controls (P < 0.05). The concentration of 8-isoPGF(2alpha) in hypothyroidism patients was similar to that in normal controls. Although the level of 8-isoPGF(2alpha) in two of the patients was slightly elevated, the P value was not significant (NS). Our data suggest that the increased level of urinary 8-isoPGF(2alpha) may reflect possible oxidation injuries in hyperthyroidism patients due to the high level of thyroid hormones. Therefore, the elevated 8-isoPGF(2alpha) in patients could be used as an important biomarker for hyperthyroidism disease. (C) 2004 Published by Elsevier Ltd.