Production of enantioselective lipase from Acinetobacter sp. SY-01

Abstract

Lipase from Acinetobacter sp. SY-01 plays an important role enzyme that products chiral drug. We investigated optimum condition for mass production of Acinetobacter sp. SY-01 lipase. Addition of among the different oils to medium. olive oil was optimal for enzyme production. When 0.2% olive oil was added as a carbon source, the production of lipase was increased to a maximum. The optimum pH and temperature were pH 7 and 30°C. In the presence of Fe 2÷ and Ca2+, the lipase activity was dramatically enhanced by 280% and 160%, respectively. SY-01 lipase was stable in the most of the DMSO among organic solvents. The addition of triton-X 100 increased the SY-01 lipase by 100-fold. The optimum composition of medium for production of the enzyme was 0.8% yeast extract, 0.2% olive oil, 0.4% triton X-100 + 40% DMSO. 0.1% NH4Cl, 0.4% K2HPO4, 3.9% NaH 2PO4, 0.03% CaCl2 · 2H2O, 0.01% FeSO4 · 7H2O (pH 7.0).