alpha-mannosidase activity from antibody raised against a glucal antigen

Abstract

Sixty cell lines of monoclonal antibody were raised against a glucal hapten 1. Among them, Ab 405.4 showed alpha-mannosidase activity as k(cat) = 0.19 /day (k(cat)/k(uncat) = 110,000). The chemical modification study and pH profile study of this antibody indicated that carboxyl group(s) in the antigen binding site involved in catalytic mechanism. (C) 1998 Elsevier Science Ltd. All rights reserved.