Introducing Protein Extraction and Preparation in Urine and Blood Samples for Doping Analysis

Abstract

Doping analysis has become a critical issue in modern society, addressing the fairness of athletes in sports and their medical well-being. Specifically, with the increasing prominence of protein pharmaceuticals among prohibited substances in doping, protein extraction and preparation in urine or blood samples play a pivotal role in determining the quality and reliability of analysis. This presentation aims to introduce the importance of protein sample preparation and various techniques employed in doping analysis. We will explore the current primary techniques for efficient protein extraction, considering the types of amino acids and matrices involved. Furthermore, it will discuss methods such as antibody-based enrichment for low-concentration proteins, enzymatic digestion for quantitative analysis, and the utilization of top-down approaches for high-resolution protein analysis. Overall, this presentation seeks to comprehensively introduce the significance of protein sample preparation in doping analysis and highlight the current major techniques, contributing to advancements in research and applications in this field.