Adhesion Behavior of Chondrocyte and Fibroblast on specific Peptide-immobilized PLGA Film
- Adhesion Behavior of Chondrocyte and Fibroblast on specific Peptide-immobilized PLGA Film
- 조성배; 박철호; 박귀덕; 정동준; 한동근
- 펩타이드; PLGA 필름; 연골세포; PLGA film; Surface modification; RGD; GVMGFO; Cell adhesion
- Issue Date
- 대한창상학회지; Journal of the Korean Wound Care Society
- VOL 5, NO 2, 88-93
- Poly (lactic-co-glycolic acid) (PLGA) has been widely used in tissue engineering because of its biocompatibility and
biodegradation. However, its hydrophobicity typically has some disadventages in the cell adhesion, proliferation, and
differentiation. Therefore, this study aims the surface modification of PLGA film using peptide grafting technology. To
achieve this purpose, plasma treatment technique was used and then RGD and GVMGFO peptides were chemically
grafted through the reactions between carboxylic acid and amine group. For the cell adhesion on these
surface-modified PLGA, chondrocytes and fibroblasts were seeded on PLGA-PAA-RGD and PLGA-PAA-GVMGFO
films, respectively and then cultured for 24 hours in incubators. The ratio of chondrocyte adhesion increased in the
order of the adhesion, PLGA＜PLGA-PAA＜PLGA-PAA-GVMGFO. Meanwhile, the number of fibroblast adhered to
PLGA-PAA-RGD films was greater than that on the PLGA-PAA-GVMGFO films. Therefore, the peptide grafting on PLGA
might be suitable to improve the cell adhesion for tissue regeneration.
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