Validation and applications of gas chromatography-combustion/isotope ratio mass spectrometric method to control misuse of androgens in human
- Validation and applications of gas chromatography-combustion/isotope ratio mass spectrometric method to control misuse of androgens in human
- 이강미; 허영란; 윤은영; 이성권; 권오승; 이재익
- Issue Date
- 2010 한국질량분석학회 여름정기학술대회
- For drug testing in sport, the abuse of anabolic androgenic steroids is prohibited by World Anti-Doping Agency (WADA) not only because it can promote performance but also because it is harmful to health. Generally, for the detection of synthetic steroids, GC-MS has been accepted. This conventional mass spectrometric method is, however, limited by their ability to identify administration of endogenous steroids due to the individual variation of steroid concentrations. Therefore, it is important to distinguish endogenous steroids from their synthetic copies which have same chemical structure with them. The measurement of 13C/12C ratio by gas chromatography-combustion /isotope ratio mass spectrometry (GC-C/IRMS) is highly regarded in doping control laboratories to elucidate the origin of androgens. The difference of 13C/12C ratio between synthetic analogues and endogenous reference compounds such as 11-keto-etiocholanolone, 11b-OH-androsterone and pregnanediol is used to confirm their synthetic origin. In this study, we validated a comprehensive GC-C/IRMS based method combined with HPLC cleanup. Intra- and inter-day precision, specificity and isotope fractionation were evaluated and the present method was accurate and reproducible. When the method was applied to WADA proficiency test and routine analyses, the results were successful.
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