ENZYMATIC INCORPORATION OF 3’-O-FLUORESCENTLY MODIFIED NUCLEOTIDES FOR SEQUENCING-BY-SYNTHESIS
- ENZYMATIC INCORPORATION OF 3’-O-FLUORESCENTLY MODIFIED NUCLEOTIDES FOR SEQUENCING-BY-SYNTHESIS
- 김다래; 이정석; 신동윤; 안대로
- Issue Date
- 2010 International Round Table on Nucleosides, Nucleotides and Nucleic Acids
- Incorporation of 3’-O-fluorescently modified nucleotides
by a DNA polymerase was examined. Among the
dye-labeled dTTPs tested, modified nucleotides with Pacific
Blue and Bodipy-FL could be completely incorporated.
Due to the blocking groups on the 3’-OH, termination
of DNA synthesis was observed after extension of
a single nucleotide in the primer. Since the linker is
chemically cleavable under DNA compatible conditions,
those modified nucleotides may be used as reversible
terminators in sequencing-by-synthesis (SBS)
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