Non-invasive imaging of gene silencing efficacy by siRNA delivery system

Non-invasive imaging of gene silencing efficacy by siRNA delivery system
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KIST-Tohoku joint symposium on nanobiomedical engineering
Gene expression control by the small interfering RNA (siRNA) is a most promising tool for gene therapy by sequence specific cleavage of mRNA. But siRNA’s instability and poor cell penetrating properties are being obstacles for its application. So, it requires effective siRNA delivery system for diverse use. Here we designed the new carrier which composed of tumor-homing chitosan based nanoparticles (NP) for complex formation with siRNA. Adopting Red Fluorescence Protein (RFP) transfected mouse melanoma B16F10 cells and athymic nude mouse, potential gene reduction effects of NP-siRNA were examined by using fluorescence imaging methods for cellular uptake, gene silencing and biodistribution. Although the development of various gene delivery systems has increased the siRNA delivery efficiency, many problems still remain to be resolved before the clinical application of siRNA. Here, we suggest reducible polymerized siRNA a possible solution for low delivery efficiency of siRNA. By increasing the size of siRNA (poly-siRNA), we obtained enhanced cellular uptake of siRNA and powerful gene silencing effect.
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